EPHA8 Antibody

Datasheet

Code	CSB-PA007728LA01HU
Size	US$166
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Image	Immunofluorescence staining of SH-SY5Y cells with CSB-PA007728LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EPHA8 Polyclonal antibody

Uniprot No.

P29322

Target Names

EPHA8

Alternative Names

EPHA8 antibody; EEK antibody; HEK3 antibody; KIAA1459Ephrin type-A receptor 8 antibody; EC 2.7.10.1 antibody; EPH- and ELK-related kinase antibody; EPH-like kinase 3 antibody; EK3 antibody; hEK3 antibody; Tyrosine-protein kinase receptor EEK antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ephrin type-A receptor 8 protein (45-249AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EPHA8 Antibody (Product code: CSB-PA007728LA01HU) is Non-conjugated. For EPHA8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA007728LB01HU	EPHA8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA007728LC01HU	EPHA8 Antibody, FITC conjugated
Biotin	CSB-PA007728LD01HU	EPHA8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system plays also a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth.

Gene References into Functions

Q-PCR and IHC staining revealed that EphA8 was highly expressed in OTSCC tissues, especially in advanced stage OTSCC tissues. Kaplan-Meier curve showed that high EphA8 expression was significantly associated with poor prognosis. PMID: 30300334
High EPHA8 expression is associated with epithelial ovarian cancer. PMID: 26989075
Human miR-10a and EphA8 regulate epithelial-mesenchymal transition (EMT) to affect glioma cell migration and invasion. PMID: 25683004
RINL, as a GEF for Rab5 subfamily, is implicated in the EphA8-degradation pathway via its interaction with odin. PMID: 22291991
role in stimulated cell migration in presnce of p110 gamma PI-3 kinase PMID: 12681484
EphA8 receptor is capable of inducing a sustained increase in MAPK activity, thereby promoting neurite outgrowth in neuronal cells PMID: 15782114

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection. Early endosome membrane.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily

Database Links

HGNC: 3391

OMIM: 176945

KEGG: hsa:2046

STRING: 9606.ENSP00000166244

UniGene: Hs.283613

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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