EPRS Antibody

Datasheet

Citations (1)

Code	CSB-PA007747LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: 293T whole cell lysate All lanes: EPRS antibody at 7.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 171 kDa Observed band size: 171 kDa IHC image of CSB-PA007747LA01HU diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA007747LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EPRS Polyclonal antibody

Uniprot No.

P07814

Target Names

EPRS

Alternative Names

Bifunctional aminoacyl tRNA synthetase antibody; Bifunctional aminoacyl-tRNA synthetase antibody; Bifunctional glutamate/proline tRNA ligase antibody; Cell proliferation-inducing gene 32 protein antibody; DKFZp313B047 antibody; EARS antibody; Eprs antibody; GLNS antibody; Glu pro tRNA synthetase antibody; GLUPRORS antibody; GluRS antibody; Glutamate tRNA ligase antibody; Glutamatyl prolyl tRNA synthetase antibody; Glutaminyl tRNA synthetase antibody; Glutamyl prolyl tRNA synthetase antibody; Glutamyl tRNA synthetase antibody; Glutamyl-tRNA synthetase antibody; PARS antibody; PIG 32 antibody; PIG32 antibody; Proliferation inducing gene 32 protein antibody; Proliferation inducing protein 32 antibody; Proline tRNA ligase antibody; Proline--tRNA ligase antibody; Prolyl tRNA synthetase antibody; Prolyl-tRNA synthetase antibody; QARS antibody; QPRS antibody; SYEP_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Bifunctional glutamate/proline--tRNA ligase protein (1207-1399AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EPRS Antibody (Product code: CSB-PA007747LA01HU) is Non-conjugated. For EPRS Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA007747LB01HU	EPRS Antibody, HRP conjugated	ELISA
FITC	CSB-PA007747LC01HU	EPRS Antibody, FITC conjugated
Biotin	CSB-PA007747LD01HU	EPRS Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Quantitative Proteomic Analysis of Zearalenone-Induced Intestinal Damage in Weaned Piglets L Ma,Toxins,2022

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample type: cells

Review: The relative abundance of proteins (APCS, PTGR1, FOLH1, EPRS, EEF2K, S100A16) between the control and ZEN groups analyzed by Western blot.

By Anonymous

Target Background

Function

Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition. Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan.

Gene References into Functions

five different EPRS mutations were identified. PMID: 29576217
Data indicate the necessity of EPRS for proliferation of tamoxifen-resistant estrogen receptor (ER+) breast cancer, but not ER- breast cancer cells. PMID: 27612429
analysis of the heterotetrameric complex structure of the glutathione transferase (GST) domains shared among the four MSC components, methionyl-tRNA synthetase (MRS), glutaminyl-prolyl-tRNA synthetase (EPRS), AIMP2 and AIMP3 PMID: 26472928
Dynamic model simulations predicted an inhibitory GAIT-element-interacting factor to account for this relationship and led to the identification of a truncated form of EPRS, a GAIT constituent that mediates binding to target transcripts. PMID: 22386318
Study reveals a unique role of Cdk5/p35 in activation of the major noncanonical function of EPRS, namely translational control of macrophage inflammatory gene expression. PMID: 21220307
Results show that glutamyl-prolyl-tRNA synthetase has a regulated, noncanonical activity that blocks synthesis of a specific protein. PMID: 15479637
Essentiality of this enzyme's domains in its noncanonical function of regulating inflammatory gene expression. PMID: 18374644
EPRS phosphorylation events regulate GAIT-mediated gene silencing. PMID: 19647514

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Subcellular Location

Cytoplasm, cytosol. Membrane; Peripheral membrane protein.

Protein Families

Class-I aminoacyl-tRNA synthetase family, Glutamate--tRNA ligase type 2 subfamily; Class-II aminoacyl-tRNA synthetase family

Database Links

HGNC: 3418

OMIM: 138295

KEGG: hsa:2058

STRING: 9606.ENSP00000355890

UniGene: Hs.497788

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