EPRS Antibody

Code CSB-PA007747LA01HU
Size US$166
Order now
Image
  • Western Blot
    Positive WB detected in: 293T whole cell lysate
    All lanes: EPRS antibody at 7.4μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 171 kDa
    Observed band size: 171 kDa

  • IHC image of CSB-PA007747LA01HU diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA007747LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) EPRS Polyclonal antibody
Uniprot No.
Target Names
EPRS
Alternative Names
Bifunctional aminoacyl tRNA synthetase antibody; Bifunctional aminoacyl-tRNA synthetase antibody; Bifunctional glutamate/proline tRNA ligase antibody; Cell proliferation-inducing gene 32 protein antibody; DKFZp313B047 antibody; EARS antibody; Eprs antibody; GLNS antibody; Glu pro tRNA synthetase antibody; GLUPRORS antibody; GluRS antibody; Glutamate tRNA ligase antibody; Glutamatyl prolyl tRNA synthetase antibody; Glutaminyl tRNA synthetase antibody; Glutamyl prolyl tRNA synthetase antibody; Glutamyl tRNA synthetase antibody; Glutamyl-tRNA synthetase antibody; PARS antibody; PIG 32 antibody; PIG32 antibody; Proliferation inducing gene 32 protein antibody; Proliferation inducing protein 32 antibody; Proline tRNA ligase antibody; Proline--tRNA ligase antibody; Prolyl tRNA synthetase antibody; Prolyl-tRNA synthetase antibody; QARS antibody; QPRS antibody; SYEP_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Bifunctional glutamate/proline--tRNA ligase protein (1207-1399AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The EPRS Antibody (Product code: CSB-PA007747LA01HU) is Non-conjugated. For EPRS Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA007747LB01HU EPRS Antibody, HRP conjugated ELISA
FITC CSB-PA007747LC01HU EPRS Antibody, FITC conjugated
Biotin CSB-PA007747LD01HU EPRS Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Applications : WB

Sample type: cells

Review: The relative abundance of proteins (APCS, PTGR1, FOLH1, EPRS, EEF2K, S100A16) between the control and ZEN groups analyzed by Western blot.

By Anonymous

Target Background

Function
Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition. Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan.
Gene References into Functions
  1. five different EPRS mutations were identified. PMID: 29576217
  2. Data indicate the necessity of EPRS for proliferation of tamoxifen-resistant estrogen receptor (ER+) breast cancer, but not ER- breast cancer cells. PMID: 27612429
  3. analysis of the heterotetrameric complex structure of the glutathione transferase (GST) domains shared among the four MSC components, methionyl-tRNA synthetase (MRS), glutaminyl-prolyl-tRNA synthetase (EPRS), AIMP2 and AIMP3 PMID: 26472928
  4. Dynamic model simulations predicted an inhibitory GAIT-element-interacting factor to account for this relationship and led to the identification of a truncated form of EPRS, a GAIT constituent that mediates binding to target transcripts. PMID: 22386318
  5. Study reveals a unique role of Cdk5/p35 in activation of the major noncanonical function of EPRS, namely translational control of macrophage inflammatory gene expression. PMID: 21220307
  6. Results show that glutamyl-prolyl-tRNA synthetase has a regulated, noncanonical activity that blocks synthesis of a specific protein. PMID: 15479637
  7. Essentiality of this enzyme's domains in its noncanonical function of regulating inflammatory gene expression. PMID: 18374644
  8. EPRS phosphorylation events regulate GAIT-mediated gene silencing. PMID: 19647514

Show More

Hide All

Subcellular Location
Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
Protein Families
Class-I aminoacyl-tRNA synthetase family, Glutamate--tRNA ligase type 2 subfamily; Class-II aminoacyl-tRNA synthetase family
Database Links

HGNC: 3418

OMIM: 138295

KEGG: hsa:2058

STRING: 9606.ENSP00000355890

UniGene: Hs.497788

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*