FBLN2 Antibody

Datasheet

Code	CSB-PA008453LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate All lanes: FBLN2 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 127, 132 kDa Observed band size: 127 kDa IHC image of CSB-PA008453LA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA008453LA01HU diluted at 1:500 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FBLN2 Polyclonal antibody

Uniprot No.

P98095

Target Names

FBLN2

Alternative Names

Fbln2 antibody; FBLN2_HUMAN antibody; FIBL-2 antibody; Fibulin-2 antibody; OTTHUMP00000216670 antibody; OTTHUMP00000216671 antibody; OTTHUMP00000216672 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Fibulin-2 protein (301-440AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FBLN2 Antibody (Product code: CSB-PA008453LA01HU) is Non-conjugated. For FBLN2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA008453LB01HU	FBLN2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA008453LC01HU	FBLN2 Antibody, FITC conjugated
Biotin	CSB-PA008453LD01HU	FBLN2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Its binding to fibronectin and some other ligands is calcium dependent. May act as an adapter that mediates the interaction between FBN1 and ELN.

Gene References into Functions

Using in vitro approaches and cultured breast cancer cell lines authors demonstrate that Fibulin-2 is a better substrate for ADAMTS-5 than it is for ADAMTS-4. Fibulin-2 degradation is associated to an enhancement of the invasive potential of T47D, MCF-7 and SK-BR-3 cells. PMID: 28099917
levels of fibulin 2 dramatically increased in the retinal pigment epithelium following retinal detachment, suggesting a direct role for fibulin 2 in the re-attachment of the retina to the retinal pigment epithelium. PMID: 24692557
Study identified and validated a novel bona fide target of miR-1, Fibullin-2 (Fbln2), a secreted protein implicated in extracellular matrix remodeling. PMID: 23612897
Two variants in the fibulin2 gene are associated with lower systolic blood pressure and decreased risk of hypertension. PMID: 22912785
Data provide evidence that FBLN2 short isoform (FBLN2S) has an important tumor-suppressive and anti-angiogenic role in nasopharyngeal carcinoma (NPC). PMID: 21743496

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Fibulin family

Tissue Specificity

Component of both basement membranes and other connective tissues. Expressed in heart, placenta and ovary.

Database Links

HGNC: 3601

OMIM: 135821

KEGG: hsa:2199

STRING: 9606.ENSP00000384169

UniGene: Hs.198862

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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