FZD8 Antibody

Datasheet

Code	CSB-PA880970LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse lung tissue, Mouse kidney tissue All lanes: FZD8 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA880970LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FZD8 Polyclonal antibody

Uniprot No.

Q9H461

Target Names

FZD8

Alternative Names

FZD8; Frizzled-8; Fz-8; hFz8

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Frizzled-8 protein (55-233AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FZD8 Antibody (Product code: CSB-PA880970LA01HU) is Non-conjugated. For FZD8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA880970LB01HU	FZD8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA880970LC01HU	FZD8 Antibody, FITC conjugated
Biotin	CSB-PA880970LD01HU	FZD8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

Gene References into Functions

FZD8 forms a TGF-beta-regulated complex with TGF-beta receptors that is mediated by the extracellular domains of FZD8 and TGFBR1 PMID: 29717114
In OS tissues, miR-520b was inversely correlated with FZD8 expression. FZD8 was upregulated in human spinal OS tissues and cell lines. PMID: 28247840
miR-375 functions as a tumor-suppressive microRNA by directly acting upon FZD8, which may serve as a new therapeutic target to inhibit tumor metastasis in colorectal cancer PMID: 27276676
Elevated expression of TP53 target FZD8 promoted prostate cancer bone metastasis by activating the canonical Wnt/beta-catenin signaling pathway. PMID: 28602974
these findings suggest that miR-100 suppresses the migration and invasion of breast cancer cells by targeting FZD-8 and inhibiting Wnt/b-catenin signaling pathway and manipulation of miR-100 may provide a promoting therapeutic strategy for cancer breast treatment. PMID: 26537584
Frizzled-8 has an important pro-inflammatory role and suggest that its expression is related to chronic bronchitis. PMID: 26797711
report the discovery of a human-accelerated regulatory enhancer (HARE5) of FZD8, a receptor of the Wnt pathway implicated in brain development and size PMID: 25702574
Study concludes that expression of Fzd8 is repressed in multiple cancers and suggest it may have a role as a tumor suppressor. PMID: 26590425
c-Met upregulated FZD8 through the ERK/c-Fos cascade in HN-CSC. Taken together, our results offer a preclinical proof-of-concept for targeting the c-Met/FZD8 signaling axis as a CSC-directed therapy to improve HNSCC treatment PMID: 25320014
These observations suggest that c-Jun is involved in APF-mediated inhibition for bladder tumor cell growth PMID: 21872498
APF has been shown to be a nine-residue frizzled-8 protein-related sialoglycopeptide. Subsequent research has gradually clarified the molecular mechanisms underlying the profound antiproliferative effect of APF. PMID: 22738385
analysis of the frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6 PMID: 20093360
molecular model of the Wnt protein binding site on the surface of dimeric CRD domain of the Fzd8 receptor PMID: 18505162

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Subcellular Location

Membrane; Multi-pass membrane protein. Golgi apparatus. Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor Fz/Smo family

Tissue Specificity

Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.

Database Links

HGNC: 4046

OMIM: 606146

KEGG: hsa:8325

STRING: 9606.ENSP00000363826

UniGene: Hs.302634

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Related pathways

Hippo signaling pathway Melanogenesis mTOR signaling pathway Wnt signaling pathway Signaling pathways regulating pluripotency of stem cells G protein coupled receptor signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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