GJD2 Antibody

Code CSB-PA891536LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: U87 whole cell lysate
    All lanes: GJD2 antibody at 3.2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • IHC image of CSB-PA891536LA01HU diluted at 1:500 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA891536LA01HU diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA891536LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GJD2 Polyclonal antibody
Uniprot No.
Target Names
GJD2
Alternative Names
GJD2; GJA9; Gap junction delta-2 protein; Connexin-36; Cx36; Gap junction alpha-9 protein
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Gap junction delta-2 protein (99-197AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The GJD2 Antibody (Product code: CSB-PA891536LA01HU) is Non-conjugated. For GJD2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA891536LB01HU GJD2 Antibody, HRP conjugated ELISA
FITC CSB-PA891536LC01HU GJD2 Antibody, FITC conjugated
Biotin CSB-PA891536LD01HU GJD2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Gene References into Functions
  1. a possible contribution of connexin 36 to amyotrophic lateral sclerosis pathogenesis PMID: 29246791
  2. Our findings suggest that the stimulatory effect of hexanol and isoflurane on Cx36 gap junction conductance could be achieved by re-shuffling of the inter-subunit disulphide bond between C264 and C92 to the intra-subunit one between C264 and C87. PMID: 29298877
  3. Our studies have shown that the heritability of myopia makes 66.4% in Lithuania. We detected significant associations between the combinations of GJD2 CC and RASGRF1 GT and odds ratio of developing myopia. PMID: 29793445
  4. Study shows that human outer retina displays a diverse cohort of connexin 36 gap junctions that follows the general mammalian scheme and display a great functional diversity. PMID: 26173976
  5. Sparse punctate Cx36 expression was seen in the myenteric plexus in nerve trunks and some platelet-derived growth factor receptor-alpha-positive cell and interstitial cells of Cajal fibers in patients with Hirschsprung's disease. PMID: 27916369
  6. Genetic variants in ZC3H11B, RSPO1, and GJD2 are associated with susceptibility to the development of high myopia in a Han Chinese population. PMID: 26485405
  7. It was shown that the decreased level of the examined neuronal proteins was accompanied by the impaired coexpression of synaptophysin/neurofilaments and Cx36 in the series of astrocytomas--anaplastic astrocytomas--glioblastomas. PMID: 26226778
  8. In this study, there was no association of the analyzed SNPs located in RASGRF1. GJD2, and ACTC1 with pathological myopia. PMID: 23834555
  9. Three SNP alleles in BRD2, Cx-36, and ME2 and microdeletions in 15q13.3, 15q11.2, and 16p13.11 also contribute risk to juvenile myclonic epilepsy. PMID: 23756480
  10. Cx36 GJs are highly cation-selective and should exhibit relatively low permeability to numerous vital negatively charged metabolites and high permeability to potassium ion, a major charge carrier in cell-cell communication. PMID: 22752717
  11. A polymorphism of Cx36 gene is associated to certain forms of human diabetes PMID: 22288100
  12. connexin genes Gjd2 coding for mCx36, Gjc1 coding for mCx45 and Gja10 coding for mCx57 in the mouse, a subset of 4 connexin genes, including the unique GJA9 (Cx59) and GJA10 (Cx62), could be detected at least as transcript isoforms in the human retina. PMID: 20979653
  13. Variations in GJD2 is associated with refractive errors and myopia. PMID: 20835239
  14. Mutations in the calcium-binding motifs of CDH23 and the 35delG mutation in GJB2 cause hearing loss in one family PMID: 12522556
  15. connexin 36 expression is regulated by the transcriptional repressor NRSF/REST PMID: 14565956
  16. 35delG mutation of the GJB2 gne is a risk for deafness PMID: 15083701
  17. Results of reporter gene analysis of Cx36 expression in transgenic mice suggest that Cx36 has functional roles not only in several types of neurons in the retina and central nervous system but also in excitable cells of the pancreas and adrenal gland. PMID: 15116387
  18. significant association between juvenile myoclonic epilepsy and a Polymorphism, Single Nucleotide within exon 2 of CX36. PMID: 15235036
  19. Intercellular coupling occurs between neuronal and microglial populations through Cx36 gap junctions; This has important implications for normal neural physiology and microglial responses in neuronopathology in the mammalian CNS. PMID: 16211561
  20. The present results provide confirmatory evidence for an allelic and genotypic association of the CX36 gene with juvenile myoclonic epilepsy. PMID: 16876983
  21. Beta cells are extensively coupled within pancreatic islets via exchanges of mostly positively charged molecules across Cx36 channels. PMID: 17828386
  22. The data show that Cx36 is a native protein of human pancreatic islets, which mediates the coupling of the insulin-producing beta-cells, and contributes to control beta-cell function by modulating gene expression. PMID: 19000992
  23. CaMKII and Cx36 were shown to be significantly colocalized in the inferior olive, a brainstem nucleus highly enriched in electrical synapses, indicating physical proximity of these proteins PMID: 19095792
  24. ZO-2 may serve to anchor regulatory proteins at gap junctions composed of Cx36. PMID: 19418635

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Protein Families
Connexin family, Delta-type subfamily
Tissue Specificity
Highly expressed in neurons.
Database Links

HGNC: 19154

OMIM: 607058

KEGG: hsa:57369

STRING: 9606.ENSP00000290374

UniGene: Hs.283816

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