GLIS2 Antibody

Datasheet

Code	CSB-PA861185LA01HU
Size	US$166
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Image	IHC image of CSB-PA861185LA01HU diluted at 1:350 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA861185LA01HU at 1:116, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GLIS2 Polyclonal antibody

Uniprot No.

Q9BZE0

Target Names

GLIS2

Alternative Names

FLJ38247 antibody; GLI similar 2 antibody; GLI-similar 2 antibody; GLIS 2 antibody; GLIS family zinc finger 2 antibody; glis2 antibody; GLIS2_HUMAN antibody; Kruppel like zinc finger protein GLIS2 antibody; Neuronal Krueppel-like protein antibody; Zinc finger protein GLIS2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Zinc finger protein GLIS2 protein (332-505AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GLIS2 Antibody (Product code: CSB-PA861185LA01HU) is Non-conjugated. For GLIS2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA861185LB01HU	GLIS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA861185LC01HU	GLIS2 Antibody, FITC conjugated
Biotin	CSB-PA861185LD01HU	GLIS2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.

Gene References into Functions

CBFA2T3-GLIS2 fusion is associated with acute megakaryoblastic leukemia in non-Down syndrome. PMID: 28063190
specific interference with ETO2-GLIS2 oligomerization reverses the transcriptional activation at enhancers and promotes megakaryocytic differentiation, providing a relevant interface to target in this poor-prognosis pediatric leukemia. PMID: 28292442
Clinical courses of pediatric patients with AMKL harboring the CBFA2T3-GLIS2 fusion gene are poor due to resistance to chemotherapies and SCT. New treatment strategies are necessary. PMID: 27094503
Nephronophthisis 7-related mutation C175R affects both localization and function of GLIS2 protein. PMID: 26374130
Glis2/NPHP7 is extensively modified by post-translational modifications PMID: 26083374
Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML. PMID: 24127550
BBS11 promotes accumulation of NPHP7, changing the properties of NPHP7. TRIM32 Modulates the Transcriptional Activities of Glis2 PMID: 24500717
Recessive mutation in GLIS2 gene is associated with Caroli disease. PMID: 23559409
Identification of a cryptic chromosome 16 inversion (inv(16)(p13.3q24.3)) in 27% of pediatric acute megakaryoblastic leukemia cases, which encodes a CBFA2T3-GLIS2 fusion protein. PMID: 23153540
Glis2 can function as a transcriptional activator and that post-translational modification within its DNA-binding domain can regulate its transcriptional activity PMID: 21127075
Characterization of Glis2, a novel gene encoding a Gli-related, Kruppel-like transcription factor with transactivation and repressor functions PMID: 11741991
Glis2 functions as a negative modulator of beta-catenin/TCF-mediated transcription. PMID: 17289029
identification of Gli-similar 2 (Glis2) as a novel binding protein for p120 catenin PMID: 17344476
Study identified Glis2 as a transcription factor mutated in Nephronophthisis and demonstrate its essential role for the maintenance of renal tissue architecture through prevention of apoptosis and fibrosis. PMID: 17618285

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Involvement in disease

Nephronophthisis 7 (NPHP7)

Subcellular Location

Nucleus speckle. Cytoplasm.

Protein Families

GLI C2H2-type zinc-finger protein family

Tissue Specificity

Expressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon.

Database Links

HGNC: 29450

OMIM: 608539

KEGG: hsa:84662

STRING: 9606.ENSP00000262366

UniGene: Hs.592087

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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