GNG2 Antibody

Datasheet

Code	CSB-PA009614OA01HU
Size	US$166
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Image	IHC image of CSB-PA009614OA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA009614OA01HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA009614OA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GNG2 Polyclonal antibody

Uniprot No.

P59768

Target Names

GNG2

Alternative Names

G gamma I antibody; G gamma-I antibody; GBG2_HUMAN antibody; GNG 2 antibody; Gng2 antibody; Guanine nucleotide binding protein gamma 2 antibody; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence from Human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 protein (44-62AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GNG2 Antibody (Product code: CSB-PA009614OA01HU) is Non-conjugated. For GNG2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA009614OB01HU	GNG2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA009614OC01HU	GNG2 Antibody, FITC conjugated
Biotin	CSB-PA009614OD01HU	GNG2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Gene References into Functions

High GNG2 expression is associated with alcoholic hepatitis. PMID: 28818508
G-protein betagamma subunits are positive regulators of Kv7.4 and native vascular Kv7 channel activity. PMID: 25941381
Alteration of gene expression profiling including GPR174 and GNG2 is associated with vasovagal syncope. PMID: 25367286
Data indicate that endogenous mTOR interacts with Gbetagamma. PMID: 24462769
increased protein expression level of GNG2 alone inhibits proliferation of malignant melanoma cells in vitro and in vivo PMID: 23031273
presence of Gng2 and Netrin-G2 immunoreactive elements in the insular cortex, but not in the putamen, suggests a possible common ontogeny of the claustrum and insula PMID: 22957104
Gbetagamma inhibits Epac-induced Ca 2+ elevation in melanoma cells. Cross talk of Ca 2+ signaling between Gbetagamma & Epac plays a major role in melanoma cell migration. PMID: 21679469
Data implicate the domain I-II linker region as an important contributor to voltage dependent Gbeta1/Ggamma2 modulation of Cav2.2 calcium channels. PMID: 20181083
Data show that activation of PLCbeta(2) by alpha(q) and beta1gamma2 differ from activation by Rac2 and from each other. PMID: 20007712
Data show that G protein inhibition of N-type calcium channels is critically dependent on two separate but adjacent approximately 20-amino acid regions of the Gbeta subunit, as examined with Gbetas 1 and 5 and Ggamma2. PMID: 15105422
10 genes were down-regulated following treatment of the T-ALL cells with 0.15 and 1.5 microg/mL of metal ores at 72 h PMID: 15747776
Fission of transport carriers at the trans-Golgi network is dependent on specifically PLCbeta3, which is necessary to activate PKCeta and PKD in that Golgi compartment, via diacylglycerol production. PMID: 17492941
signaling pathway by which G(i)-coupled receptor specifically induces Rac and Cdc42 activation through direct interaction of Gbetagamma with FLJ00018. PMID: 18045877

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Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

G protein gamma family

Tissue Specificity

Expressed in fetal tissues, including testis, adrenal gland, brain, white blood cells and brain.

Database Links

HGNC: 4404

OMIM: 606981

KEGG: hsa:54331

STRING: 9606.ENSP00000334448

UniGene: Hs.187772

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Chemokine signaling pathway PI3K-Akt signaling pathway Ras signaling pathway Apelin signaling pathway Glutamatergic synapse

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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