GPR12 Antibody

Datasheet

Code	CSB-PA009742LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate All lanes: GPR12 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa IHC image of CSB-PA009742LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA009742LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GPR12 Polyclonal antibody

Uniprot No.

P47775

Target Names

GPR12

Alternative Names

GPR12; G-protein coupled receptor 12

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human G-protein coupled receptor 12 protein (1-48AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GPR12 Antibody (Product code: CSB-PA009742LA01HU) is Non-conjugated. For GPR12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA009742LB01HU	GPR12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA009742LC01HU	GPR12 Antibody, FITC conjugated
Biotin	CSB-PA009742LD01HU	GPR12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Promotes neurite outgrowth and blocks myelin inhibition in neurons. Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production.

Gene References into Functions

Since we have demonstrated that CBD is an inverse agonist for GPR12, this provides novel mechanism of action for CBD, and an initial chemical scaffold upon which highly potent and efficacious agents acting on GPR12 may be developed with the ultimate goal of blocking cancer metastasis. PMID: 28888984
GPR12 may play a role in cell proliferation and survival PMID: 22430950
control of gene expression in vascular endothelial cells in the presence of fluid shear stress and classifies it as a sphingosine 1-phosphate receptor PMID: 12649592

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Database Links

HGNC: 4466

OMIM: 600752

KEGG: hsa:2835

STRING: 9606.ENSP00000370844

UniGene: Hs.123034

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

G protein coupled receptor signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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