GSDMA Antibody

Datasheet

Code	CSB-PA850415LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HGC27 whole cell lysate All lanes: GSDMA antibody at 3.85µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa IHC image of CSB-PA850415LA01HU diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA850415LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GSDMA Polyclonal antibody

Uniprot No.

Q96QA5

Target Names

GSDMA

Alternative Names

GSDMA antibody; GSDM antibody; GSDM1 antibody; FKSG9Gasdermin-A antibody; Gasdermin-1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Gasdermin-A protein (64-172AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GSDMA Antibody (Product code: CSB-PA850415LA01HU) is Non-conjugated. For GSDMA Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA850415LB01HU	GSDMA Antibody, HRP conjugated	ELISA
FITC	CSB-PA850415LC01HU	GSDMA Antibody, FITC conjugated
Biotin	CSB-PA850415LD01HU	GSDMA Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The product CSB-PA850415LA01HU is an IgG rabbit polyclonal GSDMA antibody suitable for the recognition of human GSDMA by ELISA, WB, IHC, and IF. The recombinant human GSDMA protein (64-172aa) is used to immunize the rabbit to generate the GSDMA polyclonal antibody. GSDMA antibody is available in the non-conjugated IgG antibody form. Its purity is over 95% using protein G purification.

The GSDMA protein act as a regulator of cell death and inflammation. GSDMA is primarily expressed in epithelial tissues, where it has been shown to play a critical role in regulating immune responses and tissue homeostasis. It also participates in various physiological processes, including skin development and maintenance of the skin barrier function. Dysregulation of GSDMA causes skin disorders, inflammatory bowel disease, cancer, and other diseases.

Customer Reviews and Q&A

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Target Background

Function

This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-A, N-terminal) binds to membranes and forms pores, triggering cell death.; Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage in vitro of genetically engineered GSDMA, and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Binds to membrane inner leaflet lipids, such as phosphatidylinositol (4,5)-bisphosphate. The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown.

Gene References into Functions

modest changes (8-13%) in promoter methylation levels of ZPBP2 and GSDMA may cause substantial changes in RNA levels and allelic expression of ZPBP2 and ORMDL3 is mediated by DNA methylation PMID: 28241063
GSDMA was identified as a susceptibility gene for systemic sclerosis. PMID: 28314753
we investigated the association between GSDMA and GSDMB variants and the incidence of adult and childhood asthma among Jordanians.An association between the GSDMB T/C single nucleotide polymorphism (SNP) genotype and the incidence of childhood asthma was found PMID: 26886240
The local genotype influences methylation levels at SLC22A5 and ZPBP2 promoters independently of the asthma status. Further studies are necessary to confirm the relationship between GSDMA-ZPBP2 and SLC22A5 methylation and asthma in females and males separately. PMID: 26671913
Based on our results and published findings on GSDMA, GSDMB, LRRC3C, and related proteins, we propose that this locus in part affects IBD susceptibility via effects on apoptosis and cell proliferation PMID: 26484354
Study identified significantly white blood cell count (WBC) level associated SNPs of three separate genes GSDMA, MED24, and PSMD3 in European continent (EA) subjects. PMID: 22037903
The GSDMA (rs7212938) and GSDMB (rs7216389) polymorphisms are associated with asthma susceptibility and intermediate asthma phenotypes. PMID: 21337730
Results suggest an association of 17q21 polymorphisms with ORMDL3, GSDMA expression, and IL-17 secretion early in life. These observations may imply a functional role of the 17q21 locus affecting T-cell development during immune maturation. PMID: 21546069
The present data suggest that TGF-beta, LMO1, possibly RUNX3, and GSDM form a regulatory pathway for directing the pit cells to apoptosis. PMID: 17471240
Study investigated the expression pattern of the GSDM family genes in the upper gastrointestinal epithelium and cancers. PMID: 19051310
Evolutionary recombination hotspot around the GSDML-GSDM locus is closely linked to oncogenomic recombination hotspot around the PPP1R1B-STARD3-TCAP-PNMT-PERLD1-ERBB2-C17orf37-GRB7 amplicon at human chromosome 17q12. PMID: 15010812

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Subcellular Location

[Gasdermin-A]: Cytoplasm, perinuclear region. Cytoplasm, cytosol.; [Gasdermin-A, N-terminal]: Cell membrane; Multi-pass membrane protein.

Protein Families

Gasdermin family

Tissue Specificity

Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and muc

Database Links

HGNC: 13311

OMIM: 611218

KEGG: hsa:284110

STRING: 9606.ENSP00000301659

UniGene: Hs.448873

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