HECTD2 Antibody

Datasheet

Code	CSB-PA732977LA01HU
Size	US$166
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Image	IHC image of CSB-PA732977LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA732977LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA732977LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HECTD2 Polyclonal antibody

Uniprot No.

Q5U5R9

Target Names

HECTD2

Alternative Names

FLJ16050 antibody; FLJ37306 antibody; HECD2_HUMAN antibody; HECT domain containing 2 antibody; HECT domain containing protein 2 antibody; HECT domain-containing protein 2 antibody; Hectd2 antibody; OTTHUMP00000020086 antibody; OTTHUMP00000038605 antibody; Probable E3 ubiquitin protein ligase HECTD2 antibody; Probable E3 ubiquitin-protein ligase HECTD2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Probable E3 ubiquitin-protein ligase HECTD2 protein (674-776AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HECTD2 Antibody (Product code: CSB-PA732977LA01HU) is Non-conjugated. For HECTD2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA732977LB01HU	HECTD2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA732977LC01HU	HECTD2 Antibody, FITC conjugated
Biotin	CSB-PA732977LD01HU	HECTD2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.; (Microbial infection) Catalyzes ubiquitination of Botulinum neurotoxin A light chain (LC) of C.botulinum neurotoxin type A (BoNT/A).

Gene References into Functions

A major biological consequence of upregulation of miR-221 is reprogramming of androgen receptor signaling via downregulation of HECTD2 and RAB1A. PMID: 23770851
HECTD2 polymorphisms are not associated with genetic susceptibility to sporadic Creutzfeldt-Jakob disease in a Korean population PMID: 21335971
HECTD2 haplotypes are associated with the susceptibility to human prion diseases. PMID: 19214206
The common haplotypes of HECTD2, tagged by rs12249854, are not associated with susceptibility to late onset Alzheimer's disease PMID: 19754925

Database Links

HGNC: 26736

KEGG: hsa:143279

STRING: 9606.ENSP00000298068

UniGene: Hs.596096

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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