Recombinant Human Probable E3 ubiquitin-protein ligase HECTD2 (HECTD2)

Code CSB-YP732977HU
MSDS
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Source Yeast
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Code CSB-EP732977HU
MSDS
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Source E.coli
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Code CSB-EP732977HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP732977HU
MSDS
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Source Baculovirus
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Code CSB-MP732977HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HECTD2
Uniprot No.
Alternative Names
FLJ16050; FLJ37306; HECD2_HUMAN; HECT domain containing 2; HECT domain containing protein 2 ; HECT domain-containing protein 2; Hectd2; OTTHUMP00000020086; OTTHUMP00000038605; Probable E3 ubiquitin protein ligase HECTD2; Probable E3 ubiquitin-protein ligase HECTD2
Species
Homo sapiens (Human)
Expression Region
1-776
Target Protein Sequence
MSEAVRVPSP ATPLVVAAPA PEERKGKESE REKLPPIVSA GAGATAGLDR GAKGQISTFS SFISAVSPKK EAAENRSSPA HLVFPNIKNV REPPPICLDV RQKQRTSMDA SSSEMKAPVL PEPILPIQPK TVKDFQEDVE KVKSSGDWKA VHDFYLTTFD SFPELNAAFK KDATASFNTI EDSGINAKFV NAVYDTLLNT PQDVQKTVLK GIINSLLREW KGPRTKDDLR AYFILLQNPQ FNNTSTYVIY AHLLRQIATL VEADHHFLVH WFKKLSQKRF KQLVERLLQF ISLRLFPAKP EEFPPITKCS WWIPSAAKVL ALLNTANNLV HPPLIPYTDF YNSTLDHIDL MEEYHTWQNF GNSHRFSFCQ YPFVISVAAK KIIIQRDSEQ QMINIARQSL VDKVSRRQRP DMNILFLNMK VRRTHLVSDS LDELTRKRAD LKKKLKVTFV GEAGLDMGGL TKEWFLLLIR QIFHPDYGMF TYHKDSHCHW FSSFKCDNYS EFRLVGILMG LAVYNSITLD IRFPPCCYKK LLSPPIIPSD QNIPVGICNV TVDDLCQIMP ELAHGLSELL SHEGNVEEDF YSTFQVFQEE FGIIKSYNLK PGGDKISVTN QNRKEYVQLY TDFLLNKSIY KQFAAFYYGF HSVCASNALM LLRPEEVEIL VCGSPDLDMH ALQRSTQYDG YAKTDLTIKY FWDVVLGFPL DLQKKLLHFT TGSDRVPVGG MADLNFKISK NETSTNCLPV AHTCFNQLCL PPYKSKKDLK QKLIIGISNS EGFGLE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.; (Microbial infection) Catalyzes ubiquitination of Botulinum neurotoxin A light chain (LC) of C.botulinum neurotoxin type A (BoNT/A).
Gene References into Functions
  1. A major biological consequence of upregulation of miR-221 is reprogramming of androgen receptor signaling via downregulation of HECTD2 and RAB1A. PMID: 23770851
  2. HECTD2 polymorphisms are not associated with genetic susceptibility to sporadic Creutzfeldt-Jakob disease in a Korean population PMID: 21335971
  3. HECTD2 haplotypes are associated with the susceptibility to human prion diseases. PMID: 19214206
  4. The common haplotypes of HECTD2, tagged by rs12249854, are not associated with susceptibility to late onset Alzheimer's disease PMID: 19754925
Database Links

HGNC: 26736

KEGG: hsa:143279

STRING: 9606.ENSP00000298068

UniGene: Hs.596096

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