HIST1H2AG Antibody

Datasheet

Code CSB-PA010389LA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P0C0S8
Image
  • Western blot
    All lanes: HIST1H2AG antibody at 4μg/ml + HL60 whole cell lysate
    Secondary
    Goat polyclonal to rabbit at 1/10000 dilution
    Predicted band size: 14 kDa
    Observed band size: 14 kDa

  • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010389LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010389LA01HU at dilution of 1:100

  • Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010389HU) against the PGK1 promoter.

  • Immunofluorescent analysis of Hela cells using CSB-PA010389LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunogen Recombinant Human Histone H2A type 1 protein (2-130AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM, H2AFP; H2AFC; H2AFD; H2AFI; H2AFN
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Others
Target Names HIST1H2AG
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2A family
Database Links

HGNC: 4737

OMIM: 602787

KEGG: hsa:8329

STRING: 9606.ENSP00000352627

UniGene: Hs.134999

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