HSD17B12 Antibody

Datasheet

Code	CSB-PA687494LA01HU
Size	US$166
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Image	Western blot All lanes: HSD17B12 antibody at 1.2µg/ml Lane 1: U251 whole cell lysate Lane 2: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 11 kDa Observed band size: 35 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA687494LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA687494LA01HU at dilution of 1:100 Immunofluorescent analysis of U251 cells using CSB-PA687494LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HSD17B12 Polyclonal antibody

Uniprot No.

Q53GQ0

Target Names

HSD17B12

Alternative Names

HSD17B12; SDR12C1; Very-long-chain 3-oxoacyl-CoA reductase; 17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase; KAR; Estradiol 17-beta-dehydrogenase 12; Short chain dehydrogenase/reductase family 12C member 1

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Very-long-chain 3-oxoacyl-CoA reductase protein (40-181AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HSD17B12 Antibody (Product code: CSB-PA687494LA01HU) is Non-conjugated. For HSD17B12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA687494LB01HU	HSD17B12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA687494LC01HU	HSD17B12 Antibody, FITC conjugated
Biotin	CSB-PA687494LD01HU	HSD17B12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.

Gene References into Functions

The expression of HSD17B12 increased along with the severity of ovarian cancer, and the expression mimicked COX-2 expression and intensity. This further suggests the involvement of HSD17B12 in AA production, and its coexpression with COX-2 indicates a role for the enzyme in the increased prostaglandin production during ovarian cancer progression. PMID: 29324448
Significant associations have been found between CpG sites and patient sex, including DNA methylation in CASP6, a gene that may respond to estradiol treatment, and in HSD17B12, which encodes a sex steroid hormone. PMID: 24058506
These evidences were suggestive of a 46,XY DSD due to 17betaHSD3 deficiency. An homozygous mutation (IVS3 -1 G>C or c.326-1G>C) of the 17betaHSD3 gene was discovered. PMID: 23435447
HSD17B12 overexpression is shown to be a marker of poor survival in patients with ovarian cancer; expression in the tumor and function of this enzyme facilitates ovarian cancer progression. PMID: 22903146
High HSD17B12 expression is associated with neoplasms. PMID: 21409596
17beta-HSD12 is the major estrogenic 17beta-HSD responsible for the conversion of E1 to E2 in women. PMID: 16166196
Location of 17beta-hydroxysteroid dehydrogenase type 12 through the human body. PMID: 16621523
17beta-HSD12 is not \related to intratumoral estradiol biosynthesis in human breast carcinoma, but is correlated with production of very long chain fatty acids and tumor progresssion. PMID: 19190350
determined the activity and expression levels of known estrogenic 17beta-HSDs, namely types 1, 7 and 12 17beta-HSD in preadipocytes before and after differentiation into mature adipocytes PMID: 19429442
There is no difference in catalytic properties between variants of 17beta-HSD types 7 and 12 and wild-type enzymes, while variants p.Glu77Gly and p.Lys183Arg in 17beta-HSD type 5 showed a slightly decreased activity. PMID: 19460435
SREBP-1 represents one of the transcriptional regulators of human 17beta-HSD12. PMID: 19533843

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family, 17-beta-HSD 3 subfamily

Tissue Specificity

Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.

Database Links

HGNC: 18646

OMIM: 609574

KEGG: hsa:51144

STRING: 9606.ENSP00000278353

UniGene: Hs.132513

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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