HIST1H3A (Ab-128) Antibody

Datasheet
Code CSB-PA010418OA128nme1HU
Size US$319
Image
  • IHC image of CSB-PA010418OA128nme1HU diluted at 1:20 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA010418OA128nme1HU diluted at 1:20 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse brain tissue, Mouse liver tissue
    All lanes: HIST1H3A antibody at 0.53µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunoprecipitating HIST1H3A in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA010418OA128nme1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA010418OA128nme1HU (5µg) + Hela whole cell lysate (500µg)
    Lane 3: Hela whole cell lysate (20µg)

Product Details

Full Product Name Rabbit anti-Human HIST1H3A Polyclonal antibody
Uniprot No. P68431
Target Names HIST1H3A
Alternative Names Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Immunogen Peptide sequence around site of Arg (128) derived from Human Histone H3.1
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:2000
IHC 1:10-1:100
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Glioma (GLM)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H3 family
Database Links

HGNC: 4766

OMIM: 137800

KEGG: hsa:8350

STRING: 9606.ENSP00000444823

UniGene: Hs.132854

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