IFIT1 Antibody

Code CSB-PA011018LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA011018LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA011018LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA011018LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, Rat liver tissue, Mouse liver tissue
    All lanes: IFIT1 antibody at 4μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 56, 52 kDa
    Observed band size: 56 kDa

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Product Details

Description
IFIT1 polyclonal antibody CSB-PA011018LA01HU was produced in the rabbits immunized by using the Recombinant Human Interferon-induced protein with tetratricopeptide repeats 1 protein (1-181AA) as the immunogen. The target protein IFIT1 specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Diseases associated with IFIT1 include hepatitis C and hepatitis.
This Rabbit anti-Homo sapiens (Human) IFIT1 Polyclonal antibody was tested in the ELISA, WB, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the IFIT1 proteins of human or mouse or rat-origin and may be used to detect the endogenous levels of IFIT1 protein.
Full Product Name Rabbit anti-Homo sapiens (Human) IFIT1 Polyclonal antibody
Uniprot No. P09914
Target Names IFIT1
Alternative Names C56 antibody; G10P1 antibody; GARG 16 antibody; GARG16 antibody; IFI 56 antibody; IFI 56K antibody; IFI-56K antibody; IFI56 antibody; IFIT-1 antibody; Ifit1 antibody; IFIT1_HUMAN antibody; IFNAI1 antibody; Interferon alpha inducible protein (MW 56kD) antibody; Interferon induced 56 kDa protein antibody; Interferon induced protein 56 antibody; Interferon inducible mRNA 561 antibody; Interferon-induced 56 kDa protein antibody; Interferon-induced protein with tetratricopeptide repeats 1 antibody; ISG56 antibody; OTTHUMP00000020062 antibody; P56 antibody; RNM561 antibody
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Immunogen Recombinant Human Interferon-induced protein with tetratricopeptide repeats 1 protein (1-181AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Applications : IHC staining

Sample type: human cells

Review: The protein contents and distribution of IFIT1 and IFIT3 were examined in nonmetastatic HCC tissues, metastatic HCC tissues, and para-carcinoma tissues by IHC staining.

By Anonymous

Target Background

Function
(From Uniprot)
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
Gene References into Functions
  1. Induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection. PMID: 29629559
  2. Human IFIT1 and mouse IFIT1B have divergent antiviral specificities: only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. PMID: 27240734
  3. Taken together, this study revealed that IFIT1 played an important antiviral role in human cytomegalovirus infected fetal astrocytes. PMID: 27589693
  4. Glioblastoma patients with high IFIT1 and low MGMT expression have an improved prognosis. PMID: 26980050
  5. IFIT1 significantly inhibited human parainfluenza virus type 3, whereas IFIT2, IFIT3, and IFIT5 were less effective or not at all. PMID: 27707917
  6. All the rubulaviruses tested were sensitive to the antiviral action of ISG56/IFIT1. PMID: 27512068
  7. IFIT1 is involved in the regulation of IFNalpha treatment for chronic hepatitis B and its polymorphism rs303218 can predict the end point virological response. PMID: 27956805
  8. IFIT1 is not a dominant restriction factor against three different families of negative-sense RNA viruses. PMID: 26157117
  9. Reovirus T3D infection induced STAT-1, ISG-15, IFIT-1, Mx1, and IFIT-3 expression. PMID: 25905045
  10. protein expression is inhibited by hepatitis C virus PMID: 23529855
  11. The specificity of IFIT1 for 2'O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2'O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells. PMID: 24098121
  12. TLR4 signaling, induced by LPS, increases the expression of melanoma differentiation-associated gene 5 (MDA5) and interferon PMID: 23684765
  13. ISG56 may play a role in immune and inflammatory reactions induced by toll-like receptor 3 signaling in human mesangial cells. PMID: 23363937
  14. Results indicate that miR-203 is an interferon-inducible miRNA that can negatively regulate a number of cellular mRNAs, including an interferon-stimulated gene target, IFIT1/ISG56, by destabilizing its mRNA transcript. PMID: 23785202
  15. Data suggested that PIV5 mRNAs are methylated at the 2'-hydroxyl group and that ISG56/IFIT1 inhibits the translation of PIV5 mRNA by some unrecognized mechanism. Also ISG56/IFIT1 is primarily responsible for the IFN-induced inhibition of PIV5. PMID: 23052390
  16. crystal structures of human IFIT5, its complex with PPP-RNAs, and an amino-terminal fragment of IFIT1 PMID: 23334420
  17. Hepatitis C virus infection suppresses the upregulation of a subset of effector molecules, including ISG56 and IFITM1. PMID: 21976647
  18. ISG56 interacts with ribosomal protein L15 in gastric cancer cells. PMID: 21612406
  19. Expression of ifi56 is enhanced significantly when the differentiation of APL cells was induced by all-trans retinoic acid. PMID: 21129252
  20. IFIT1 bound PPP-RNA antagonizes viruses by sequestering specific viral nucleic acids. PMID: 21642987
  21. The authors present evidence that P56 (ISG56 protein), the expression of which is strongly induced by IFN, double-stranded RNA and viruses, mediates the anti-HPV effect of IFN. PMID: 19008854
  22. ISG56 is a mediator of negative-feedback regulation of virus-triggered induction of type I IFNs and cellular antiviral responses PMID: 19416887

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Subcellular Location Cytoplasm.
Protein Families IFIT family
Database Links

HGNC: 5407

OMIM: 147690

KEGG: hsa:3434

STRING: 9606.ENSP00000360869

UniGene: Hs.20315

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