Product Details

Uniprot No.

Target Names

IFIT1

Alternative Names

C56 antibody; G10P1 antibody; GARG 16 antibody; GARG16 antibody; IFI 56 antibody; IFI 56K antibody; IFI-56K antibody; IFI56 antibody; IFIT-1 antibody; Ifit1 antibody; IFIT1_HUMAN antibody; IFNAI1 antibody; Interferon alpha inducible protein (MW 56kD) antibody; Interferon induced 56 kDa protein antibody; Interferon induced protein 56 antibody; Interferon inducible mRNA 561 antibody; Interferon-induced 56 kDa protein antibody; Interferon-induced protein with tetratricopeptide repeats 1 antibody; ISG56 antibody; OTTHUMP00000020062 antibody; P56 antibody; RNM561 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from the N-terminal region of Human IFI-56K.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:40000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.

Gene References into Functions

Induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection. PMID: 29629559
Human IFIT1 and mouse IFIT1B have divergent antiviral specificities: only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. PMID: 27240734
Taken together, this study revealed that IFIT1 played an important antiviral role in human cytomegalovirus infected fetal astrocytes. PMID: 27589693
Glioblastoma patients with high IFIT1 and low MGMT expression have an improved prognosis. PMID: 26980050
IFIT1 significantly inhibited human parainfluenza virus type 3, whereas IFIT2, IFIT3, and IFIT5 were less effective or not at all. PMID: 27707917
All the rubulaviruses tested were sensitive to the antiviral action of ISG56/IFIT1. PMID: 27512068
IFIT1 is involved in the regulation of IFNalpha treatment for chronic hepatitis B and its polymorphism rs303218 can predict the end point virological response. PMID: 27956805
IFIT1 is not a dominant restriction factor against three different families of negative-sense RNA viruses. PMID: 26157117
Reovirus T3D infection induced STAT-1, ISG-15, IFIT-1, Mx1, and IFIT-3 expression. PMID: 25905045
protein expression is inhibited by hepatitis C virus PMID: 23529855
The specificity of IFIT1 for 2'O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2'O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells. PMID: 24098121
TLR4 signaling, induced by LPS, increases the expression of melanoma differentiation-associated gene 5 (MDA5) and interferon PMID: 23684765
ISG56 may play a role in immune and inflammatory reactions induced by toll-like receptor 3 signaling in human mesangial cells. PMID: 23363937
Results indicate that miR-203 is an interferon-inducible miRNA that can negatively regulate a number of cellular mRNAs, including an interferon-stimulated gene target, IFIT1/ISG56, by destabilizing its mRNA transcript. PMID: 23785202
Data suggested that PIV5 mRNAs are methylated at the 2'-hydroxyl group and that ISG56/IFIT1 inhibits the translation of PIV5 mRNA by some unrecognized mechanism. Also ISG56/IFIT1 is primarily responsible for the IFN-induced inhibition of PIV5. PMID: 23052390
crystal structures of human IFIT5, its complex with PPP-RNAs, and an amino-terminal fragment of IFIT1 PMID: 23334420
Hepatitis C virus infection suppresses the upregulation of a subset of effector molecules, including ISG56 and IFITM1. PMID: 21976647
ISG56 interacts with ribosomal protein L15 in gastric cancer cells. PMID: 21612406
Expression of ifi56 is enhanced significantly when the differentiation of APL cells was induced by all-trans retinoic acid. PMID: 21129252
IFIT1 bound PPP-RNA antagonizes viruses by sequestering specific viral nucleic acids. PMID: 21642987
The authors present evidence that P56 (ISG56 protein), the expression of which is strongly induced by IFN, double-stranded RNA and viruses, mediates the anti-HPV effect of IFN. PMID: 19008854
ISG56 is a mediator of negative-feedback regulation of virus-triggered induction of type I IFNs and cellular antiviral responses PMID: 19416887