Product Details

Target Name

interferon-induced protein with tetratricopeptide repeats 1

Alternative Names

C56 ELISA Kit; G10P1 ELISA Kit; GARG 16 ELISA Kit; GARG16 ELISA Kit; IFI 56 ELISA Kit; IFI 56K ELISA Kit; IFI-56K ELISA Kit; IFI56 ELISA Kit; IFIT-1 ELISA Kit; Ifit1 ELISA Kit; IFIT1_HUMAN ELISA Kit; IFNAI1 ELISA Kit; Interferon alpha inducible protein (MW 56kD) ELISA Kit; Interferon induced 56 kDa protein ELISA Kit; Interferon induced protein 56 ELISA Kit; Interferon inducible mRNA 561 ELISA Kit; Interferon-induced 56 kDa protein ELISA Kit; Interferon-induced protein with tetratricopeptide repeats 1 ELISA Kit; ISG56 ELISA Kit; OTTHUMP00000020062 ELISA Kit; P56 ELISA Kit; RNM561 ELISA Kit

Abbreviation

IFIT1

Uniprot No.

P09914

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IFIT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	98
	Range %	93-103
1:4	Average %	88
	Range %	84-92
1:8	Average %	102
	Range %	97-108

Recovery

The recovery of human IFIT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-105
EDTA plasma (n=4)	89	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.835	2.986	2.911	2.770
500	2.183	2.295	2.239	2.098
250	1.321	1.397	1.359	1.218
125	0.702	0.714	0.708	0.567
62.5	0.453	0.435	0.444	0.303
31.25	0.277	0.285	0.281	0.140
15.6	0.229	0.224	0.227	0.086
0	0.140	0.142	0.141

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human IFIT1 ELISA Kit was designed for the quantitative measurement of Human IFIT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Background

Function
(From Uniprot)

Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.

Gene References into Functions

Induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection. PMID: 29629559
Human IFIT1 and mouse IFIT1B have divergent antiviral specificities: only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. PMID: 27240734
Taken together, this study revealed that IFIT1 played an important antiviral role in human cytomegalovirus infected fetal astrocytes. PMID: 27589693
Glioblastoma patients with high IFIT1 and low MGMT expression have an improved prognosis. PMID: 26980050
IFIT1 significantly inhibited human parainfluenza virus type 3, whereas IFIT2, IFIT3, and IFIT5 were less effective or not at all. PMID: 27707917
All the rubulaviruses tested were sensitive to the antiviral action of ISG56/IFIT1. PMID: 27512068
IFIT1 is involved in the regulation of IFNalpha treatment for chronic hepatitis B and its polymorphism rs303218 can predict the end point virological response. PMID: 27956805
IFIT1 is not a dominant restriction factor against three different families of negative-sense RNA viruses. PMID: 26157117
Reovirus T3D infection induced STAT-1, ISG-15, IFIT-1, Mx1, and IFIT-3 expression. PMID: 25905045
protein expression is inhibited by hepatitis C virus PMID: 23529855
The specificity of IFIT1 for 2'O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2'O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells. PMID: 24098121
TLR4 signaling, induced by LPS, increases the expression of melanoma differentiation-associated gene 5 (MDA5) and interferon PMID: 23684765
ISG56 may play a role in immune and inflammatory reactions induced by toll-like receptor 3 signaling in human mesangial cells. PMID: 23363937
Results indicate that miR-203 is an interferon-inducible miRNA that can negatively regulate a number of cellular mRNAs, including an interferon-stimulated gene target, IFIT1/ISG56, by destabilizing its mRNA transcript. PMID: 23785202
Data suggested that PIV5 mRNAs are methylated at the 2'-hydroxyl group and that ISG56/IFIT1 inhibits the translation of PIV5 mRNA by some unrecognized mechanism. Also ISG56/IFIT1 is primarily responsible for the IFN-induced inhibition of PIV5. PMID: 23052390
crystal structures of human IFIT5, its complex with PPP-RNAs, and an amino-terminal fragment of IFIT1 PMID: 23334420
Hepatitis C virus infection suppresses the upregulation of a subset of effector molecules, including ISG56 and IFITM1. PMID: 21976647
ISG56 interacts with ribosomal protein L15 in gastric cancer cells. PMID: 21612406
Expression of ifi56 is enhanced significantly when the differentiation of APL cells was induced by all-trans retinoic acid. PMID: 21129252
IFIT1 bound PPP-RNA antagonizes viruses by sequestering specific viral nucleic acids. PMID: 21642987
The authors present evidence that P56 (ISG56 protein), the expression of which is strongly induced by IFN, double-stranded RNA and viruses, mediates the anti-HPV effect of IFN. PMID: 19008854
ISG56 is a mediator of negative-feedback regulation of virus-triggered induction of type I IFNs and cellular antiviral responses PMID: 19416887

Hide All

Subcellular Location

Cytoplasm.

Protein Families

IFIT family

Database Links

HGNC: 5407

OMIM: 147690

KEGG: hsa:3434

STRING: 9606.ENSP00000360869

UniGene: Hs.20315

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Human Interferon-induced protein with tetratricopeptide repeats 1(IFIT1) ELISA kit

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Target Background