IFITM2 Antibody

Code CSB-PA011025LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
    All lanes: IFITM2 antibody at 4.7µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 15 kDa
    Observed band size: 15 kDa

  • IHC image of CSB-PA011025LA01HU diluted at 1:700 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescent analysis of HepG2 cells using CSB-PA011025LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Description

The immunization of rabbits with the recombinant human IFITM2 protein (1-56AA) and final purification from rabbit antiserum by protein G causes the creation of the anti-IFITM2 antibody. This IFITM2 antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95%. It is only reactive with human IFITM2 protein, an IFN-stimulated effector that has a board-spectrum antiviral activity against diverse viruses. And this anti-IFITM2 antibody has been validated for use in ELISA, WB, IHC, and IF applications.

Full Product Name Rabbit anti-Homo sapiens (Human) IFITM2 Polyclonal antibody
Uniprot No. Q01629
Target Names IFITM2
Alternative Names IFITM2; Interferon-induced transmembrane protein 2; Dispanin subfamily A member 2c; DSPA2c; Interferon-inducible protein 1-8D
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Interferon-induced transmembrane protein 2 protein (1-56AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
IF 1:50-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Applications : WB

Sample type: Human PAM cell

Review: Western blot analysis of IFITMs in PAM cell lines stably expressing Flag-tagged IFITM1, IFITM2, or IFITM3 or CMV alone using an IFITM1-specific antibody and IFITM2-specific antibody against IFITM1 and IFITM2, respectively.

By Anonymous

Target Background

Function
(From Uniprot)
IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV and SARS-CoV-2 S protein-mediated viral entry and VSV G protein-mediated viral entry. Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation. IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome.
Gene References into Functions
  1. Delta20 IFITM2 may serve as a major contributor to the gatekeeping mechanism that explains restriction of X4 viruses in the early stage of HIV-1 infection PMID: 28630320
  2. These results indicate that IFITM2 protein can restrict alphavirus infection by inhibiting viral fusion with cellular membranes. PMID: 27219333
  3. IFITM2 promotes gastric cancer growth and metastasis via IGF1/IGF1R/STAT3 signaling pathway. PMID: 28223169
  4. Single Nucleotide Polymorphisms in 1-8D is associated with neoplasms. PMID: 19544527
Subcellular Location Cell membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein. Late endosome membrane; Single-pass type II membrane protein.
Protein Families CD225/Dispanin family
Database Links

HGNC: 5413

OMIM: 605578

KEGG: hsa:10581

STRING: 9606.ENSP00000382714

UniGene: Hs.709321

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