IFITM2 Antibody

Datasheet

Code	CSB-PA011025LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: IFITM2 antibody at 4.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa IHC image of CSB-PA011025LA01HU diluted at 1:700 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of HepG2 cells using CSB-PA011025LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) IFITM2 Polyclonal antibody

Uniprot No.

Q01629

Target Names

IFITM2

Alternative Names

IFITM2; Interferon-induced transmembrane protein 2; Dispanin subfamily A member 2c; DSPA2c; Interferon-inducible protein 1-8D

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Interferon-induced transmembrane protein 2 protein (1-56AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The IFITM2 Antibody (Product code: CSB-PA011025LA01HU) is Non-conjugated. For IFITM2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA011025LB01HU	IFITM2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA011025LC01HU	IFITM2 Antibody, FITC conjugated
Biotin	CSB-PA011025LD01HU	IFITM2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The immunization of rabbits with the recombinant human IFITM2 protein (1-56AA) and final purification from rabbit antiserum by protein G causes the creation of the anti-IFITM2 antibody. This IFITM2 antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95%. It is only reactive with human IFITM2 protein, an IFN-stimulated effector that has a board-spectrum antiviral activity against diverse viruses. And this anti-IFITM2 antibody has been validated for use in ELISA, WB, IHC, and IF applications.

Citations

Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection Li C, et al,Viruses,2019

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample type: Human PAM cell

Review: Western blot analysis of IFITMs in PAM cell lines stably expressing Flag-tagged IFITM1, IFITM2, or IFITM3 or CMV alone using an IFITM1-specific antibody and IFITM2-specific antibody against IFITM1 and IFITM2, respectively.

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Target Background

Function

IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV and SARS-CoV-2 S protein-mediated viral entry and VSV G protein-mediated viral entry. Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation. IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome.

Gene References into Functions

Delta20 IFITM2 may serve as a major contributor to the gatekeeping mechanism that explains restriction of X4 viruses in the early stage of HIV-1 infection PMID: 28630320
These results indicate that IFITM2 protein can restrict alphavirus infection by inhibiting viral fusion with cellular membranes. PMID: 27219333
IFITM2 promotes gastric cancer growth and metastasis via IGF1/IGF1R/STAT3 signaling pathway. PMID: 28223169
Single Nucleotide Polymorphisms in 1-8D is associated with neoplasms. PMID: 19544527

Subcellular Location

Cell membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein. Late endosome membrane; Single-pass type II membrane protein.

Protein Families

CD225/Dispanin family

Database Links

HGNC: 5413

OMIM: 605578

KEGG: hsa:10581

STRING: 9606.ENSP00000382714

UniGene: Hs.709321

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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