IFT46 Antibody

Code CSB-PA873621LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: MCF7 whole cell lysate, PC-3 whole cell lysate, SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse Sketetal Muscle tissue lysate, Mouse Brain tissue lysate
    All lanes: ZDHHC12 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 35/41 kDa
    Observed band size: 55 kDa
  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA873621LA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA873621LA01HU at dilution of 1:100
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) IFT46 Polyclonal antibody
Uniprot No.
Target Names
IFT46
Alternative Names
IFT46 antibody; C11orf2 antibody; C11orf60Intraflagellar transport protein 46 homolog antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Intraflagellar transport protein 46 homolog protein (1-304AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The IFT46 Antibody (Product code: CSB-PA873621LA01HU) is Non-conjugated. For IFT46 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA873621LB01HU IFT46 Antibody, HRP conjugated ELISA
FITC CSB-PA873621LC01HU IFT46 Antibody, FITC conjugated
Biotin CSB-PA873621LD01HU IFT46 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Applications : Immunoblotting analysis

Sample type: human

Review: The protein level of TTBK2, BBS4, SEPTIN2, and IFT46 was dramatically decreased along with the gradient increased ORF10 expression. Immunoblotting analysis of TTBK2, BBS4, SEPTIN2, IFT46, and ORF10 levels in ORF10-expressing and empty vector (NC) control HEK293T cells. GAPDH served as a loading control (J). Odyssey-based quantification of protein levels in J (n = 3 independent experiments). Data are presented as the mean ± SEM. Statistical analysis was performed with two-tailed unpaired Student’s t test (K). Cells transfected with pcDNA-ORF10-HA display reduced level of multiple cilium-related proteins. Immunoblotting analysis of TTBK2, BBS4, SEPTIN2, IFT46, and ORF10 levels in ORF10-expressing and empty vector (NC) control HEK293T cells. GAPDH served as a loading control (L) .

By Anonymous

Target Background

Function
Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis.
Gene References into Functions
  1. the ciliary protein IFT46 has a specific function in chondrocytes and is also essential for normal development of vertebrates. PMID: 17720815
Subcellular Location
Cytoplasm, cytoskeleton, cilium basal body. Cell projection, cilium.
Protein Families
IFT46 family
Database Links

HGNC: 26146

KEGG: hsa:56912

UniGene: Hs.533738

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