IRF5 Antibody

Code CSB-PA011820LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western Blot
    Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, SH-SY5Y whole cell lysate
    All lanes: IRF5 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 57, 58, 55, 48, 18 kDa
    Observed band size: 57 kDa

  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA011820LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA011820LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) IRF5 Polyclonal antibody
Uniprot No. Q13568
Target Names IRF5
Alternative Names Interferon regulatory factor 5 antibody; Interferon regulatory factor 5 bone marrow variant antibody; IRF 5 antibody; IRF-5 antibody; Irf5 antibody; IRF5_HUMAN antibody; SLEB10 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Interferon regulatory factor 5 protein (214-395AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma. ND Ebelt,Front Immunol,2020

Applications: Western Blot
Antibody dilution factor: 1:500
Sample type: Cell lines
Review: Western blots of tumor lysates from Day 8 Aza and DMSO-treated tumor using anti-DNMT1, anti-IRF5, and anti-survivin antibodies. GAPDH is shown as a loading control. DNMT1 and survivin were assayed using the same blot. Relative quantifications of band intensity normalized to GAPDH are shown to the right of blots.
PMID: 32296439

Target Data

Function Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.
Gene References into Functions
  1. A possible cross-reaction between IRF5/EBV homologous antigens and shifts in T cell balance disrupted by anti-IL-2 Abs. PMID: 29379122
  2. study suggests a novel role for interferon regulatory factor 5 (IRF5) in the regulation of the inflammatory response in human myometrium PMID: 30006439
  3. In IAV-infected patients, miR-302a expression was down-regulated, whereas IRF-5 expression was up-regulated. Taken together, this work uncovers and defines a signaling pathway implicated in an IAV-induced cytokine storm. PMID: 29046356
  4. Analysis of clinical HCC specimens supports a pathologic role for IRF5 in HCV-induced HCC, as IRF5 expression was down-regulated in livers from HCV-positive versus HCV-negative HCC patients or healthy donor livers. These results identify IRF5 as an important suppressor of HCV replication and HCC pathogenesis. PMID: 29079574
  5. Results indicated that IFR5 variants rs77571059 and rs2004640 and haplotype GTAA were associated with the susceptibility to CAP and rs77571059 was related to the severity of the disease, suggesting that IFR5 variants may contribute to the pathogenesis of community-acquired pneumonia (CAP), and may serve as prognostic markers of CAP susceptibility and outcome. PMID: 30176312
  6. IRF5 and its related inflammatory cytokines are associated with the severity, prognosis, and causative pathogen of CAP patients. PMID: 29847542
  7. this study shows that interferon regulatory factor 5 regulates the expression of matrix metalloproteinase-3 in human chondrocytes PMID: 29274624
  8. IFR5 variants may contribute as a host factor in determining the pathogenesis in chronic HBV infections. PMID: 29375210
  9. The presence of the interferon regulatory factor 5 rs2004640 T allele increases the risk of nephritis development in Egyptian children with systemic lupus erythematosus. PMID: 28059021
  10. IRF5 was an adverse independent prognostic factor for both overall survival and recurrence free survival in patients with non-metastatic ccRCC. PMID: 28562332
  11. Data suggest that IRAK4 activity regulates activation of IRF5, TAK1, and IKKB in monocytes; IRAK4 activation of TAK1-IKKB-IRF5 axis leads to induction of cytokines and interferons following TLR7/TLR8 stimulation. (IRAK4 = interleukin-1 receptor-associated kinase 4; IRF5 = interferon regulatory factor-5; TAK1 = MAPK kinase kinase 7; IKKB = I-kappa B kinase; TLR = toll-like receptor) PMID: 28924041
  12. IRF5 and IRF5 disease-risk variants increase glycolysis and human m1 macrophage polarization by regulating proximal signaling and Akt2 activation. PMID: 27545875
  13. the genomic pattern according to MATH demonstrated that mutation rates of TP53, IRF5 and KRAS were independently associated with MATH, and the latter two were only significant in male patients. As MATH increased, the fraction of somatic copy number alteration (SCNA) elevated. Moreover, more SCNA events was independently associated with MATH in male than in female PMID: 28531253
  14. IRF-5 targeted the expression of vascular cell adhesion molecule 1 (VCAM-1) at the transcriptional level by binding to its promoter. PMID: 28818665
  15. oncogenic IRF5 overexpression in HL is the result of a specific LTR transcriptional activation PMID: 26279299
  16. the primary activating regulatory region of human IRF5 was located in its minimal promoter region between nucleotides -179 and +62. In addition, it was shown that Sp1 was able to bind to the multiple sites in IRF5 promoter region, and was involved in the transcriptional regulation of IRF5 at the basal level. PMID: 27484157
  17. This study demonstrates the association of IRF5 with an increased susceptibility for systemic lupus erythematosus (SLE) in the population of Crete and emphasizes the association of the Neanderthal-derived IRF5 haplotype with SLE susceptibility. Patients carrying allele the Neanderthal allele C had greater type I IFN, supporting a functional consequence of this polymorphism. PMID: 28185859
  18. this study shows that IRF5 polymorphisms are associated with unexplained recurrent pregnancy loss among Iranian women PMID: 27798840
  19. The results demonstrated that STAT4 rs7574865 and IRF5 rs2004640G/T substitution are associated with a susceptibility to systemic sclerosis. PMID: 26712637
  20. IRF5 and PADI4 significantly discriminated between RA patients and healthy controls in LDA. PMID: 27092776
  21. IRF5 and IRF8, two transcription factors with opposing functions, control TLR9 signaling in human plasmacytoid dendritic cells. PMID: 26613957
  22. meta-analysis demonstrated the IRF5 rs2070197 polymorphism conferred susceptibility to systemic lupus erythematosus (SLE) in all subjects without inter-study heterogeneity; IRF5 rs2070197 polymorphism was identified as risk factors for SLE in Caucasian populations but it had no effects in Asians PMID: 26233721
  23. Genetic associations and gene-gene interactions of IRF5 and TYK2 were significantly detected in Han Chinese with systemic lupus erythematosus PMID: 26294277
  24. four single nucleotide polymorphisms studied in IRF5 may affect neither Neuromyelitis optica nor Multiple sclerosis in the Southeastern Han Chinese population PMID: 26112714
  25. h Epstein-Barr virus and Mycobacterium avium subsp. paratuberculosis epitopes of IRF5 elicit a consistent humoral response in multiple sclerosis patients PMID: 25392335
  26. Study indicates that high-level IRF-5 expression is specific to HTLV-1-infected T cells and regulated by Tax viral protein. PMID: 26004104
  27. the data demonstrate a critical mechanism by which sex differences in basal plasmacytoid dendritic cell IRF5 expression lead to higher IFN-alpha production upon TLR7 stimulation in females. PMID: 26519527
  28. regulates CXCL13-mediated recruitment of B and T cells to IRF5-positive tumor-conditioned media PMID: 25533286
  29. The polymorphisms in the IRF5 gene may play a role in the development of Crohn's Disease in the Malaysian population, as seen in the significant associations of SNPs, that is, rs3807306, rs10954213 and rs11770589. PMID: 25564941
  30. study gives no definite evidence for a pathogenic genetic link of periodontitis and RA but suggests IRF5 and PRDM1 as shared susceptibility factors. PMID: 25263394
  31. IRF5-TNPO3 genetic variation is associated systemic lupus erythematosus. PMID: 25205108
  32. A conserved region within interferon regulatory factor 5 controls breast cancer cell migration through a cytoplasmic and transcription-independent mechanism. PMID: 25649192
  33. IRF-5 is suppressed by hypermethylation of its promoter-A in most of EBV-infected transformed cells, especially Burkitt's lymphomas and EBV-associated gastric carcinoma (EBVaGC) PMID: 25557482
  34. We have found that three polymorphisms in linkage disequilibrium with the protective haplotypes of IRF5 have differential allele effects PMID: 23941291
  35. IRF5rs2004640 T (mutant) allele may be a susceptibility factor conferring risk for RA in South Indian Tamils, whereas G allele (wild type) may be protective. PMID: 25284481
  36. The association of IRF5 markers (rs2004640, rs10954213) with susceptibility to SLE and to the development of more than one autoimmune disease (OP) and to analyse the influence of rs2004640 on IRF5 isoform expression were explored PMID: 24697591
  37. TNFAIP3 and IRF5 polymorphisms were associated with polymyositis/dermatomyositis in Chinese Han patients with interstitial lung disease. PMID: 25337792
  38. gene variants in IRF5, IRF8 and GPC5 were not associated with risk of relapse or disease progression in multiple sclerosis PMID: 24943672
  39. Our results reveal that IRF5 gene variants are associated with risk of cardiovascular events in patients with Rheumatoid arthritis PMID: 25011482
  40. IKKbeta is an IRF5 kinase that instigates inflammation PMID: 25326420
  41. IKKbeta activates two "master" transcription factors of the innate immune system, IRF5 and NF-kappaB PMID: 25326418
  42. IRF5 is a key regulator in classical Hodgkin lymphoma, according to transcription factor motifs PMID: 25288773
  43. TRIpartite motif 21 (TRIM21) differentially regulates the stability of interferon regulatory factor 5 (IRF5) isoforms. PMID: 25084355
  44. The IRF5's promoter polymorphisms alter first exon usage and increase transcription levels and high levels of IRF5 may bias the immune system toward autoimmunity. PMID: 24350899
  45. Polymorphisms of IRF5 may play an important role in susceptibility to systemic sclerosis. PMID: 25572744
  46. Meta-analysis certified that IRF5 polymorphisms confer susceptibility to systemic lupus erythematous, multiple sclerosis, and systemic sclerosis. PMID: 25036352
  47. results indicate that T3-bound TRs interfere with transactivation by TEADs via protein-protein interactions, resulting in the negative regulation of MYH7 promoter activity PMID: 24788560
  48. Unlike other studies, this study found no association of the rs2004640 SNP with SLE among Han Chinese. It did however interact with SNPS in ETS1, STAT4, and IKZF1. PMID: 24697319
  49. No significant association was found between all IRF5 SNPs (rs10954213, rs2004640, rs3807306, rs752637 and rs7808907) and ophthalmopathy in Graves' patients. PMID: 24582590
  50. This meta-analysis suggests that the A allele of rs10954213 and TCA haplotype (rs2004640-rs2070197-rs10954213) in IRF5 is associated with the increased risk of SLE in different ethnic groups, and its prevalence is ethnicity dependent. PMID: 23392701
  51. Studies indicate that polymorphisms of the interferon regulatory factor 5 (IRF5) gene in can result in risk or protective haplotypes with regard to Systemic Lupus Erythematosus (SLE). susceptibility PMID: 24928322
  52. Results show that two functional genetic variants of regulatory factor 5 (IRF5) may play a role in the development of macular edema in non-anterior uveitis patients. PMID: 24116155
  53. IRF5 rs10954213G is significantly increased in MPO-ANCA-positive vasculitis patients. PMID: 23985571
  54. A haplotype carrying two functional SNPs of IRF5, rs10954213A and rs2280714A, was associated with the risk of sarcoidosis in the Japanese population. PMID: 23288367
  55. Our data identify IRF5 as a downstream target of the BCR-ABL kinase, suggesting that its biological inactivation contributes to leukemic transformation. PMID: 24445143
  56. meta-analysis. The results suggest that the IRF5 rs2004640 polymorphism is associated with rheumatoid arthritis especially when the dominant genetic model is applied. PMID: 23801380
  57. The IRF5 could significantly affect these immune cells participating in the pathogenesis of SLE, and numerous studies implied that this transcription factor is mechanistically linked to the disease progression. PMID: 23883595
  58. A redundant interaction between IRF5 and STAT4 polymorphisms was found in susceptibility to the type I interferon pathway-associated rheumatic autoimmune diseases, systemic lupus erythematosus and rheumatoid arthritis. PMID: 24014567
  59. Vpx expression results in decreased promoter binding activity of IRF5 PMID: 24532789
  60. Single nucleotide polymorphisms in IRF5, but not TLR4, DEFEB1, or VDR, are associated with the risk of ulcerative colitis in a Han Chinese population. PMID: 23971939
  61. Data show that cyclosporin A (CsA) inhibited rotavirus replication and restored interferon-beta (IFN-beta) expression, and promoted Interferon Regulatory Factor-5 (IRF-5) expression in a HT-29 cell line model. PMID: 23990993
  62. Results show that interferon regulatory factor-5 (IRF-5) and NF-kappaB p50 are key co-regulators of IFN-beta and IL-6 expression following TLR9-mediated activation of human plasmacytoid dendritic cells PMID: 23616277
  63. DNA methylation patterns of the promoter region of IRF5 to were investigated determine whether its epigenetic deregulation could explain the increased expression of IRF5 mRNA in primary Sjogren's syndrome patients. PMID: 23302156
  64. The systemic lupus erythematosus IRF5 risk haplotype is associated with systemic sclerosis. PMID: 23372721
  65. Results from this study provide the first proof that SLE patients express an IRF5 transcript signature that is distinct from healthy donors; an IRF5-SLE risk haplotype defines the top four most abundant IRF5 transcripts expressed in SLE patients. PMID: 23349905
  66. The IRF5 rs2004640, rs729302 and rs752637 polymorphisms are associated with rheumatoid arthritis susceptibility. [Meta-analysis] PMID: 23073787
  67. gene-gene epistatic interactions revealed between IRF5 and IKZF1 in systemic lupus erythematosus. PMID: 23236436
  68. study is the first to analyze associations between melanoma immune responsiveness and IRF5 polymorphism. The results support a common genetic basis which may underline the development of autoimmunity and melanoma immune responsiveness PMID: 22909381
  69. By participating in transcription complex with other co-factors, IRF5 and STAT4 harbour the potential of regulating a large number of target genes, which may contribute to their strong association with systemic lupus erythematosus PMID: 22730365
  70. These data suggest that IRF5 polymorphisms play a role in serologic autoimmunity in humans and may promote the progression to clinical autoimmunity. PMID: 22674082
  71. in thyroid malignancies, IRF5 displays tumor-promoting rather than tumor-suppressor activities. PMID: 22507190
  72. A 4-fold downregulation of IRF5 was seen in splenic marginal-zone lymphomas with 7q32 deletion vs non-deleted tumours. Ectopic expression of IRF5 decreased proliferation & increased apoptosis in vitro, & impaired lymphoma development in vivo. PMID: 22816737
  73. IRF5 polymorphisms contribute to the risk profile for Crohn's disease and ulcerative colitis. PMID: 22257839
  74. One known genetic factor associated with lupus is a haplotype of the IRF5 gene. Gene expression microarray data identified multiple interferon- and inflammation-related gene sets significantly overrepresented in cells with the risk haplotype. PMID: 22500098
  75. IRF5 and IRF7 are key transcription factors in interferon pathway that determine viral sensitivity of lung cancer cells. PMID: 22194884
  76. IRF5 polymorphism predicts prognosis in patients with systemic sclerosis. PMID: 22440820
  77. IRF5 disease-associated polymorphisms to a wide spectrum of microbial triggers has broad implications for global immunological responses, host defenses against pathogens, and inflammatory/autoimmune disease susceptibility. PMID: 22544929
  78. This study provides the first formal proof that IRF-5 activation is altered in the monocytes of systemic lupus erythematosus patients. PMID: 21968701
  79. IRF5 is an important tumor suppressor that regulates multiple cellular processes involved in the conversion of normal mammary epithelial cells to tumor epithelial cells with metastatic potential. PMID: 22053985
  80. Interferon regulatory factor 5 gene polymorphism confers risk to several rheumatic diseases and correlates with expression of alternative thymic transcripts. PMID: 22179739
  81. genetic polymorphism is associated with susceptibility to hemophagocytic lymphohistiocytosis in children PMID: 21898142
  82. The data supports the concept that IRF5 functional genetic elements contribute to SLE risk through their relationship with auto-antibodies and IFNalpha production. PMID: 22088620
  83. Although the systemic lupus erythematosus risk haplotype of IRF5 is associated with high expression of the gene, cis-regulation of IRF5 expression is not enough to fully account for IRF5 association with susceptibility. PMID: 21627826
  84. Two interferon regulatory factor 5 (IRF5) gene variants were examined for association with rheumatoid arthritis and were relevant for RA susceptibility, especially for seronegative RA. PMID: 21807777
  85. suggested that IRF5 gene polymorphism was associated with systemic lupus erythematosus in multiple ethnic populations PMID: 21834935
  86. These findings suggest a role for IRF5 gene variation in the pharmacological and clinical outcome of IFNbeta therapy that might have relevance as biomarker to predict the response to IFNbeta in multiple sclerosis. PMID: 21471993
  87. The cellular IRF5 and vIRF3 interact, leading to the functional modulation of IRF5-mediated type I IFN expression and cell cycle regulation. PMID: 21133648
  88. Data suggest that IRF5 gene polymorphism is a genetic factor influencing susceptibility to macrophage activation syndrome (MAS) in patients with systemic juvenile idiopathic arthritis, and IRF5 contributes to the pathogenesis of MAS in these patients. PMID: 21239750
  89. IRF5 exon 6 structural variants that occur as part of a systemic lupus erythematosus (SLE) risk haplotypes modulate IRF5 function. PMID: 20695768
  90. IRF5 gene confers only a modest increase in the susceptibility to multiple sclerosis, but this association is relevant as it involves a pathway that may be correlated with a therapeutic intervention. PMID: 20861862
  91. This study provides the 'association and linkage proof' establishing IRF5 as a rheumatoid arthritis susceptibility gene and the identification of a genetic factor that seems to contribute to the modulation of the erosive phenotype. PMID: 20980283
  92. Suggest that the rs2004640 T allele was associated with susceptibility to lupus nephritis and that the IRF5 polymorphism analyzed did not seem to be implicated in the pathology and clinical manifestation of lupus nephritis in the Chinese population. PMID: 21040166
  93. These data provide the first evidence whereby IRF5 regulates proinflammatory cytokine expression in conjunction with HATs and HDACs. PMID: 20935208
  94. studies found that IRF5, STAT4 and BLK are associated not only with systemic lupus erythematosus, but also rheumatoid arthritis and systemic sclerosis [review] PMID: 20453440
  95. Our study provides further evidence of a key role of IRF5 in severity of systemic sclerosis. PMID: 20231204
  96. These results implicate that epigenetic dysregulation of the IRF5 promoter is unlikely to be associated with inflammatory bowel diseases. PMID: 20127100
  97. a IRF5 haplotype is associated with reduced risk to Wegener's granulomatosis PMID: 20049410
  98. Systematic bioinformatic and biochemical analyses of the TNF gene locus map 2 sites of IRF5 recruitment: 5' upstream and 3' downstream of the TNF gene. PMID: 20237317
  99. Single nucleotide polymorphisms of IRF5 in healthy individuals of a number of ethnic groups were associated with increased mRNA expression of IRF5. PMID: 19854706
  100. There is an association of promoter indel in IRF5 with systemic lupus erythematosus in 5 different populations, and rs10954213 is the main single-nucleotide polymorphism responsible for altered IRF5 expression in peripheral blood mononuclear cells. PMID: 20080916

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Involvement in disease Inflammatory bowel disease 14 (IBD14); Systemic lupus erythematosus 10 (SLEB10); Rheumatoid arthritis (RA)
Subcellular Location Cytoplasm, Nucleus
Protein Families IRF family
Database Links

HGNC: 6120

OMIM: 180300

KEGG: hsa:3663

STRING: 9606.ENSP00000349770

UniGene: Hs.521181

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