KMT5B Antibody

Datasheet

Code	CSB-PA674569LA01HU
Size	$297
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Image	Western Blot Positive WB detected in: HT29 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), SK-BR-3 whole cell lysate(30µg), HepG2 whole cell lysate(30µg) All lanes: KMT5B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 100, 45,32 kDa Observed band size: 100 kDa Exposure time: 120s IHC image of CSB-PA674569LA01HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) KMT5B Polyclonal antibody

Uniprot No.

Q4FZB7

Target Names

KMT5B

Alternative Names

CGI 85 antibody; CGI85 antibody; Histone-lysine N-methyltransferase KMT5B antibody; Kmt5b antibody; KMT5B_HUMAN antibody; Lysine N-methyltransferase 5B antibody; Lysine-specific methyltransferase 5B antibody; MGC118906 antibody; MGC118909 antibody; MGC21161 antibody; MGC703 antibody; Su(var)4-20 homolog 1 antibody; Suppressor of variegation 4 20 homolog 1 antibody; Suppressor of variegation 4-20 homolog 1 antibody; Suv4-20h1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

A synthetic peptide of human SUV420H1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The KMT5B Antibody (Product code: CSB-PA674569LA01HU) is Non-conjugated. For KMT5B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA674569LB01HU	KMT5B Antibody, HRP conjugated	ELISA
FITC	CSB-PA674569LC01HU	KMT5B Antibody, FITC conjugated
Biotin	CSB-PA674569LD01HU	KMT5B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 50% glycerol, pH7.4.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Plays a role in myogenesis by regulating the expression of target genes, such as EID3. Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4.

Gene References into Functions

epsilon-globin expression is regulated by SUV4-20h1. PMID: 26802048
Altogether, these results reveal Suv4-20h-mediated histone H4K20 tri-methylation as a critical determinant in the selection of active replication initiation sites in heterochromatin regions of mammalian genomes. PMID: 28778956
overexpression of SUV420H1 may result in activation of the ERK signaling pathway PMID: 26586479
Strong statistical evidence for a causal role of SUV420H1 in autism. PMID: 26235986
This study suggests a novel role of FRG1 as epigenetic regulator of muscle differentiation and indicates that Suv4-20h1 has a gene-specific function in myogenesis. PMID: 23720823
The crystal structure of SUV420H1 was used to characterize substrate selectivity and product specificity. PMID: 24396869
SUV420H1 and SUV420H2 isoforms have different in their cellular localization and effects on myogenic differentiation PMID: 21206904
The data indicate that Suv4-20 generates nearly ubiquitous dimethylation that facilitates the DNA damage response and selective trimethylation that is involved in heterochromatin formation.[Suv4-20] PMID: 18296440

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar4-20 subfamily

Database Links

HGNC: 24283

OMIM: 610881

KEGG: hsa:51111

STRING: 9606.ENSP00000305899

UniGene: Hs.632120

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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