KTN1 Antibody

Datasheet

Code	CSB-PA773030LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: KTN1 antibody at 3.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 157, 150, 151, 153 kDa Observed band size: 157 kDa IHC image of CSB-PA773030LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA773030LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating KTN1 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA773030LA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA773030LA01HU (6μg) + A549 whole cell lysate (1mg) Lane 3: A549 whole cell lysate (20μg)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) KTN1 Polyclonal antibody

Uniprot No.

Q86UP2

Target Names

KTN1

Alternative Names

CG-1 antigen antibody; CG1 antibody; CG1 antigen antibody; KIAA0004 antibody; Kinectin antibody; Kinesin receptor antibody; Ktn1 antibody; KTN1_HUMAN antibody; MU-RMS-40.19 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Kinectin protein (978-1116AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The KTN1 Antibody (Product code: CSB-PA773030LA01HU) is Non-conjugated. For KTN1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA773030LB01HU	KTN1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA773030LC01HU	KTN1 Antibody, FITC conjugated
Biotin	CSB-PA773030LD01HU	KTN1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.

Gene References into Functions

Significant association between rs12895072 and rs12434554 within the KTN1 gene and accelerated decline in Cognition performance in Chinese elderly male Gout population. PMID: 28953682
Our results show that kinectin-dependent ER distribution can be localized by chemoattractants and provide a mechanism for biased protrusion choices during chemotaxis in shallow gradients of chemoattractants. PMID: 27221621
Kinectin is concentrated in the perinuclear sheets of the endoplasmic reticulum by interacting with polysomes. Kinectin may play a role in determinining the morphology of the endoplasmic reticulum. PMID: 21111237
Kinectin may have the ability to anchor mRNAs to the surface of the endoplasmic reticulum. PMID: 22679391
The kinesin-1 deficiency weakened intercellular adhesion, despite the maintenance of adherens junctions and other desmosome components at the plasma membrane. PMID: 22184201
The genes identified KTN1, ROCK1, and ZAK may be responsible for loss of cellular homeostasis in giant cells tumors of bone PMID: 21305317
kinectin is markedly enriched at integrin-based adhesion complexes, induced by clustering integrins with fibronectin-coated beads PMID: 11973345
The data suggests that both KNT and KNTvd4(-) participate in microtubule-dependent secretion of amylin in islet beta-cells. PMID: 16890462
anchoring the elongation factor-1 complex onto endoplasmic reticulum via EF-1delta/kinectin interaction is important for regulating protein synthesis in eukaryotic cells PMID: 16950774
When kinesin binds with both heads to the microtubule, the neck linkers in the rear and forward heads extend forward and backward. This supports the notion that neck linker movements accompany the 'hand-over-hand' motion of the two motor domains. PMID: 17013387
A large number of variations were found in many of the genes (myozenin 1, gamma-filamin, kinectin-1) in patients with limb-girdle muscular dystrophies and controls. PMID: 19472918
The presence of coiled-coil domains in the ktn1/ret fusion protein (PTC8) suggests ligand-independent dimerization and thus constitutive activation of the ret tyrosine kinase domain. PMID: 10850414

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Note=Vesicle membrane protein anchored to the endoplasmic reticulum.

Protein Families

Kinectin family

Tissue Specificity

High levels in peripheral blood lymphocytes, testis and ovary, lower levels in spleen, thymus, prostate, small intestine and colon.

Database Links

HGNC: 6467

OMIM: 600381

KEGG: hsa:3895

STRING: 9606.ENSP00000378720

UniGene: Hs.509414

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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