LAS1L Antibody

Datasheet

Code	CSB-PA897285LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: 293 whole cell lysate All lanes: LAS1L antibody at 3.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84, 82, 77, 34 kDa Observed band size: 84 kDa Immunofluorescence staining of HepG2 cells with CSB-PA897285LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LAS1L Polyclonal antibody

Uniprot No.

Q9Y4W2

Target Names

LAS1L

Alternative Names

dJ475B7.2 antibody; FLJ12525 antibody; FLJ30133 antibody; FLJ45274 antibody; LAS1 like (S. cerevisiae) antibody; Las1 like antibody; LAS1L antibody; LAS1L_HUMAN antibody; Protein LAS1 homolog antibody; Ribosomal biogenesis protein LAS1L antibody; RP3 475B7.2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ribosomal biogenesis protein LAS1L protein (490-565AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LAS1L Antibody (Product code: CSB-PA897285LA01HU) is Non-conjugated. For LAS1L Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA897285LB01HU	LAS1L Antibody, HRP conjugated	ELISA
FITC	CSB-PA897285LC01HU	LAS1L Antibody, FITC conjugated
Biotin	CSB-PA897285LD01HU	LAS1L Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.

Gene References into Functions

LAS1L interacts with PELP1, TEX10, and WDR18, the mammalian homologues of the budding yeast Rix1 complex, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit. PMID: 22190735
Analysis of high-molecular-weight RNAs confirmed that Las1L is required for ITS2 processing,which separates the 5.8S and 25S/28S rRNAs, as 32S was found to accumulate and 12S to diminish upon Las1L depletion. PMID: 22083961
Data demonstrate that Las1L is essential for cell proliferation and biogenesis of the 60S ribosomal subunit. PMID: 20647540

Involvement in disease

Wilson-Turner X-linked mental retardation syndrome (WTS)

Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm.

Protein Families

LAS1 family

Database Links

HGNC: 25726

OMIM: 300964

KEGG: hsa:81887

STRING: 9606.ENSP00000363944

UniGene: Hs.522675

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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