LIMD2 Antibody

Datasheet

Code	CSB-PA012950LA01HU
Size	US$166
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Image	IHC image of CSB-PA012950LA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA012950LA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of SH-SY5Y cells with CSB-PA012950LA01HU at 1:167, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LIMD2 Polyclonal antibody

Uniprot No.

Q9BT23

Target Names

LIMD2

Alternative Names

LIM domain containing 2 antibody; LIM domain-containing protein 2 antibody; Limd2 antibody; LIMD2_HUMAN antibody; MGC10986 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human LIM domain-containing protein 2 protein (1-127AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LIMD2 Antibody (Product code: CSB-PA012950LA01HU) is Non-conjugated. For LIMD2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA012950LB01HU	LIMD2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA012950LC01HU	LIMD2 Antibody, FITC conjugated
Biotin	CSB-PA012950LD01HU	LIMD2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Acts as an activator of the protein-kinase ILK, thereby regulating cell motility.

Gene References into Functions

Our data suggest that LIMD2 may play an important role in the metastatic process of papillary thyroid carcinoma PMID: 29560564

Subcellular Location

Cytoplasm. Nucleus.

Database Links

HGNC: 28142

KEGG: hsa:80774

UniGene: Hs.591166

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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