LPAR4 Antibody

Datasheet

Code	CSB-PA013050LA01HU
Size	US$166
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Image	IHC image of CSB-PA013050LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Western blot All lanes: LPAR4 antibody at 4µg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa Immunofluorescent analysis of HepG2 cells using CSB-PA013050LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LPAR4 Polyclonal antibody

Uniprot No.

Q99677

Target Names

LPAR4

Alternative Names

LPAR4; GPR23; LPA4; P2RY9; Lysophosphatidic acid receptor 4; LPA receptor 4; LPA-4; G-protein coupled receptor 23; P2Y purinoceptor 9; P2Y9; P2Y5-like receptor; Purinergic receptor 9

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Lysophosphatidic acid receptor 4 protein (1-43AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LPAR4 Antibody (Product code: CSB-PA013050LA01HU) is Non-conjugated. For LPAR4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA013050LB01HU	LPAR4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA013050LC01HU	LPAR4 Antibody, FITC conjugated
Biotin	CSB-PA013050LD01HU	LPAR4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA.

Gene References into Functions

Mutation in LPAR4 gene is associated with papillary thyroid carcinoma. PMID: 26941397
These results suggest that the diverse roles of LPA4, LPA5 and LPA6 are involved in the activation of tumor progression in pancreatic cancer cells. PMID: 25849892
Our data suggest that LPA4 signaling potentially modulates malignant behavior of SQ-20B cells. PMID: 23467751
LPA4 gene mutation may play some role in the pathogenesis of colon cancer. PMID: 20890765
LPA4 and LPA5 receptors induce osteoblastic differentiation of human mesenchymal stem cells PMID: 20069565
identified as the fourth lysophosphatidic acid (LPA) receptor; shares only 20-24% amino acid identities with Edg-2/LPA1, Edg-4/LPA2, and Edg-7/LPA3, and phylogenetic analysis also shows that p2y9/GPR23 is far distant from the Edg family PMID: 12724320

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus.

Database Links

HGNC: 4478

OMIM: 300086

KEGG: hsa:2846

STRING: 9606.ENSP00000362398

UniGene: Hs.522701

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PI3K-Akt signaling pathway Oxidative Phosphorylation Phospholipase D signaling pathway Rap1 signaling pathway G protein coupled receptor signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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