LPAR5 Antibody

Datasheet
Code CSB-PA013051OA01HU
Size US$299
Uniprot No. Q9H1C0
Image
  • IHC image of CSB-PA013051OA01HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA013051OA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Lysophosphatidic acid receptor 5 protein (310-326AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
Relevance Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Lysophosphatidic acid receptor 5 (LPA receptor 5) (LPA-5) (G-protein coupled receptor 92) (G-protein coupled receptor 93), LPAR5, GPR92 GPR93
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names LPAR5
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities.
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Not expressed in frontal cortex, basal forebrain, caudate putamen, thalamus, or hippocampus.
Database Links

HGNC: 13307

OMIM: 606926

KEGG: hsa:57121

STRING: 9606.ENSP00000327875

UniGene: Hs.155538

Pathway PI3K-Akt signaling pathway
Oxidative Phosphorylation
Phospholipase D signaling pathway
Rap1 signaling pathway

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