LRRFIP2 Antibody

Datasheet

Code	CSB-PA896921LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate All lanes: LRRFIP2 antibody at 4.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83, 46, 12, 49, 58 kDa Observed band size: 83, 49 kDa IHC image of CSB-PA896921LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA896921LA01HU diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LRRFIP2 Polyclonal antibody

Uniprot No.

Q9Y608

Target Names

LRRFIP2

Alternative Names

5133400F20Rik antibody; AI850587 antibody; DKFZp434H2035 antibody; FLJ20248 antibody; FLJ22683 antibody; FLJ58304 antibody; HUFI 2 antibody; Leucine rich repeat (in FLII) interacting protein 2 antibody; Leucine rich repeat flightless interacting protein 2 antibody; Leucine-rich repeat flightless-interacting protein 2 antibody; LRR FLII interacting protein 2 antibody; LRR FLII-interacting protein 2 antibody; LRRF2_HUMAN antibody; Lrrfip2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Leucine-rich repeat flightless-interacting protein 2 protein (211-342AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LRRFIP2 Antibody (Product code: CSB-PA896921LA01HU) is Non-conjugated. For LRRFIP2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA896921LB01HU	LRRFIP2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA896921LC01HU	LRRFIP2 Antibody, FITC conjugated
Biotin	CSB-PA896921LD01HU	LRRFIP2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta-catenin. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.

Gene References into Functions

Data show that the ability of Ca(2+) to accentuate the activity of NLRP3 inflammasome is abrogated in Flightless-I (FliI) and leucine-rich repeat FliI-interaction protein 2 (LRRFIP2)-knockdown macrophages. PMID: 27431477
FATylation of LRRFIP2 occurs on two distinct sites, each being modified by a single FAT10 moiety. PMID: 23036196
A novel exonic rearrangement affecting MLH1 and the contiguous LRRFIP2 is a founder mutation in Portuguese Lynch syndrome families PMID: 21785361
Unambiguous characterization of site-specific phosphorylation of leucine-rich repeat Fli-I-interacting protein 2 (LRRFIP2) in Toll-like receptor 4 (TLR4)-mediated signaling. PMID: 21220426
These data suggest that LRRFIP2 plays an important role in transducing Wnt signals. PMID: 15677333
LRR-binding MyD88 interactor LRRFIP2 is a positive regulator of NF-kappa B activity and is also a positive regulator of cytokine production in lipopolysaccharide-stimulated macrophages, suggesting a functional role in TLR4-mediated inflammatory response. PMID: 19265123

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Protein Families

LRRFIP family

Tissue Specificity

Widely expressed, with highest levels in heart and skeletal muscle.

Database Links

HGNC: 6703

OMIM: 614043

KEGG: hsa:9209

STRING: 9606.ENSP00000338727

UniGene: Hs.740522

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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