MED8 Antibody

Code CSB-PA006810
Size US$100
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  • Western Blot analysis of JK cells using Med8 Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
MED8
Alternative Names
Activator recruited cofactor 32 kDa component antibody; Activator-recruited cofactor 32 kDa component antibody; ARC32 antibody; med8 antibody; MED8_HUMAN antibody; Mediator complex subunit 8 antibody; Mediator of RNA polymerase II transcription subunit 8 antibody; Mediator of RNA polymerase II transcription subunit 8 homolog antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthesized peptide derived from the Internal region of Human Med8.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
Gene References into Functions
  1. mammalian mediator subunit mMED8 is an elongin BC-interacting protein that can assemble with Cul2 and Rbx1 reconstitute a ubiquitin ligase (Ubc5; E2 ubiquitin-conjugating enzyme Ubc5) PMID: 12149480
  2. ability to inhibit both host transcription and the interferon response is linked to interaction of NSs with the MED8 component of Mediator, a protein complex necessary for mRNA production PMID: 16973571
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 8 family
Database Links

HGNC: 19971

OMIM: 607956

KEGG: hsa:112950

STRING: 9606.ENSP00000290663

UniGene: Hs.301756

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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