MEGF8 Antibody

Code CSB-PA773606LA01HU
Size US$166
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Image
  • IHC image of CSB-PA773606LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA773606LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MEGF8 Polyclonal antibody
Uniprot No.
Target Names
MEGF8
Alternative Names
MEGF8 antibody; C19orf49 antibody; EGFL4 antibody; KIAA0817 antibody; Multiple epidermal growth factor-like domains protein 8 antibody; Multiple EGF-like domains protein 8 antibody; Epidermal growth factor-like protein 4 antibody; EGF-like protein 4 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Multiple epidermal growth factor-like domains protein 8 protein (495-663AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MEGF8 Antibody (Product code: CSB-PA773606LA01HU) is Non-conjugated. For MEGF8 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA773606LB01HU MEGF8 Antibody, HRP conjugated ELISA
FITC CSB-PA773606LC01HU MEGF8 Antibody, FITC conjugated
Biotin CSB-PA773606LD01HU MEGF8 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Acts as a negative regulator of hedgehog signaling.
Gene References into Functions
  1. mutations in MEGF8 cause a Carpenter syndrome subtype frequently associated with defective left-right patterning, probably through perturbation of signaling by hedgehog and nodal family members. PMID: 23063620
Involvement in disease
Carpenter syndrome 2 (CRPT2)
Subcellular Location
Membrane; Single-pass type I membrane protein.
Database Links

HGNC: 3233

OMIM: 604267

KEGG: hsa:1954

STRING: 9606.ENSP00000334219

UniGene: Hs.132483

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