MEX3B Antibody

Datasheet
Code CSB-PA751152LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q6ZN04
Image
  • IHC image of CSB-PA751152LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA751152LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of SH-SY5Y cells with CSB-PA751152LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human RNA-binding protein MEX3B protein (253-375AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200
Relevance RNA-binding protein. May be involved in post-transcriptional regulatory mechanisms.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias RNA-binding protein MEX3B (RING finger and KH domain-containing protein 3) (RING finger protein 195), MEX3B, KIAA2009 RKHD3 RNF195
Immunogen Species Human
Research Area Signal Transduction
Target Names MEX3B
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function RNA-binding protein. May be involved in post-transcriptional regulatory mechanisms.
Subcellular Location Nucleus, Cytoplasm, Cytoplasm, P-body, Cytoplasmic granule
Tissue Specificity Highest levels found in fetal brain and testis. Detected in the adult intestinal epithelium, specifically in goblet cell at protein level.
Database Links

HGNC: 25297

OMIM: 611008

KEGG: hsa:84206

STRING: 9606.ENSP00000329918

UniGene: Hs.104744

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