MEX3B Antibody

Datasheet

Code	CSB-PA751152LA01HU
Size	US$166
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Image	IHC image of CSB-PA751152LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA751152LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of SH-SY5Y cells with CSB-PA751152LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MEX3B Polyclonal antibody

Uniprot No.

Q6ZN04

Target Names

MEX3B

Alternative Names

MEX3B antibody; KIAA2009 antibody; RKHD3 antibody; RNF195RNA-binding protein MEX3B antibody; RING finger and KH domain-containing protein 3 antibody; RING finger protein 195 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human RNA-binding protein MEX3B protein (253-375AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MEX3B Antibody (Product code: CSB-PA751152LA01HU) is Non-conjugated. For MEX3B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA751152LB01HU	MEX3B Antibody, HRP conjugated	ELISA
FITC	CSB-PA751152LC01HU	MEX3B Antibody, FITC conjugated
Biotin	CSB-PA751152LD01HU	MEX3B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

RNA-binding protein. May be involved in post-transcriptional regulatory mechanisms.

Gene References into Functions

Mex3b role in the Runx3 degradation in gastric cancer. PMID: 29417297
14-3-3 exerts combined effects on hMex-3B and acts as a major regulator of the sorting between distinct classes of RNA granules PMID: 18779327

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, P-body. Cytoplasmic granule. Note=Predominantly expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. Localization to P-bodies is dependent on 14-3-3.

Tissue Specificity

Highest levels found in fetal brain and testis. Detected in the adult intestinal epithelium, specifically in goblet cell at protein level.

Database Links

HGNC: 25297

OMIM: 611008

KEGG: hsa:84206

STRING: 9606.ENSP00000329918

UniGene: Hs.104744

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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