MKL2 Antibody

Datasheet

Code	CSB-PA891962LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate All lanes: MKL2 antibody at 4.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 119, 43, 52, 115, 120 kDa Observed band size: 119 kDa IHC image of CSB-PA891962LA01HU diluted at 1:500 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MKL2 Polyclonal antibody

Uniprot No.

Q9ULH7

Target Names

MKL2

Alternative Names

FLJ31823 antibody; Megakaryoblastic leukemia 2 antibody; megakaryoblastic leukemia 2 protein antibody; MKL/myocardin-like 2 antibody; MKL/myocardin-like protein 2 antibody; Mkl2 antibody; MKL2_HUMAN antibody; MRTF-B antibody; Myocardin-related transcription factor B antibody; NPD001 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human MKL/myocardin-like protein 2 protein (164-313AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MKL2 Antibody (Product code: CSB-PA891962LA01HU) is Non-conjugated. For MKL2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA891962LB01HU	MKL2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891962LC01HU	MKL2 Antibody, FITC conjugated
Biotin	CSB-PA891962LD01HU	MKL2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.

Gene References into Functions

Taken together, these findings suggest that MKL1 and MKL2 are present at synapses and involved in dendritic spine maturation. PMID: 29335431
Study showed that MRTF-A and MRTF-B were upregulated in pancreatic cancer tissues supporting the hypothesis that both of them are oncogenes in pancreatic cancer. PMID: 26498848
While disruption of the MKL2:SRF axis has been associated with severe microcephaly and disordered brain development in multiple model systems, the role of this transcription factor complex has not been previously demonstrated in human brain development. PMID: 23692340
There were multiple independent HIV integrations in several genes, including MKL2 and BACH2; many of these integrations were in clonally expanded cells. PMID: 24968937
MKL1/2 depletion resulted in Ras activation, elevated p16 expression and hypophosphorylation of the retinoblastoma (Rb) protein in DLC1-deficient hepatocellular carcinoma cells. PMID: 23853104
Based on a recurrent translocation t(11;16)(q13;p13), the C11orf95-MKL2 fusion gene has been found in eight further cases of chondroid lipomas. PMID: 23672313
study provides evidence that MKL1/2 mediates cancerous transformation in DLC1-deficient hepatocellular and mammary carcinoma cells PMID: 22139079
C11orf95-MKL2 is the resulting fusion oncogene of t(11;16)(q13;p13) in chondroid lipoma. PMID: 20607705
dominant negative MKL2 blocked differentiation-induced expression of SRF target genes skeletal alpha-actin and alpha-myosin heavy chain and blocked differentiation of the myoblasts to myotubes in vitro. PMID: 14565952
BMP signaling modulates VSMC phenotype via cross-talk with the RhoA/MRTFs pathway, and may contribute to the development of the pathological characteristics observed in patients with PAH and other obliterative vascular diseases. PMID: 17947237
Myocardin-related transcription factors are critical mediators of transforming growth factor beta (TGF-beta) 1-induced epithelial-mesenchymal transition.[ PMID: 18056415
RNA interference was used to investigate the contribution of the MRTF-SRF pathway to cytoskeletal dynamics in MDA-MB-231 breast carcinoma and B16F2 melanoma cells, in which basal MRTF-SRF activity is Rho-dependent. PMID: 19198601

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Involvement in disease

A chromosomal aberration involving C11orf95 is found in 3 chondroid lipomas. Translocation t(11;16)(q13;p13) with C11orf95 produces a C11orf95-MKL2 fusion protein (PubMed:20607705).

Subcellular Location

Nucleus.

Database Links

HGNC: 29819

OMIM: 609463

KEGG: hsa:57496

STRING: 9606.ENSP00000339086

UniGene: Hs.49143

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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