MTMR3 Antibody

Datasheet

Code	CSB-PA622675HA01HU
Size	US$166
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Image	IHC image of CSB-PA622675HA01HU diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA622675HA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MTMR3 Polyclonal antibody

Uniprot No.

Q13615

Target Names

MTMR3

Alternative Names

FYVE domain-containing dual specificity protein phosphatase 1 antibody; FYVE-DSP1 antibody; MTMR3 antibody; MTMR3_HUMAN antibody; Myotubularin-related protein 3 antibody; Zinc finger FYVE domain-containing protein 10 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Myotubularin-related protein 3 protein (652-899AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MTMR3 Antibody (Product code: CSB-PA622675HA01HU) is Non-conjugated. For MTMR3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA622675HB01HU	MTMR3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA622675HC01HU	MTMR3 Antibody, FITC conjugated
Biotin	CSB-PA622675HD01HU	MTMR3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. May also dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues.

Gene References into Functions

Data suggest MTMR3 has multiple functions in regulation of autophagy; one mechanism appears to be direct interaction between MTMR3 and mTORC1 (MTOR, mLST8, raptor) which inhibits phosphorylation activity of mTORC1; MTMR3 is localized to Golgi apparatus. PMID: 26787466
the rs713875 IBD risk polymorphism increases MTMR3 expression, which modulates pattern recognition receptor (PRR)-induced outcomes, including increased induced caspase-1 activation PMID: 26240347
myotubularin-related protein 3 and myotubularin-related protein 4 may act as a bridge between CEP55 and polo-like kinase 1, ensuring proper CEP55 phosphorylation and regulating CEP55 recruitment to the midbody. PMID: 25659891
cloning, expression, purification and crystallization of human myotubularin-related protein 3 encompassing the PH-GRAM and the phosphatase catalytic domain are reported PMID: 25195900
These results indicate that MTMR3 may play an important role in the progression of CRC and suggest that siRNA mediated silencing of MTMR3 could be an effective tool in CRC treatment. PMID: 25215329
Our results suggest that the MTMR3 gene is shared between IgA nephropathy and lupus nephritis in the northern Chinese population, further highlighting the role of autophagy in systemic lupus erythematosus. PMID: 24943867
Data suggest MTMR3, PIKFYVE (phosphoinositide kinase FYVE), and their product phosphatidylinositol 5-phosphate are involved in activation of RAC1 (rho family small GTP binding protein); this process regulates migration/invasion of carcinoma/sarcoma. PMID: 24840251
Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. PMID: 23154468
MTMR3 belongs to the myotubularin family of phosphoinositides phosphatases PMID: 9736772
MTMR3 shares similar phosphatase activity and substrate specificity with its homologous proteins MTM1 and MTMR3 PMID: 11846405
A phylogenetic study revealing co-evolution of myotubularins with PI 3-kinase class III complex PMID: 18774718
Autophagosome formation was triggered by the overexpression of a dominant-negative inactive mutant of Myotubularin-related phosphatase 3 (MTMR3). PMID: 20059746
analysis of MTMR3 phosphoinositide binding domain properties PMID: 15840652

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Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein.

Protein Families

Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily

Database Links

HGNC: 7451

OMIM: 603558

KEGG: hsa:8897

STRING: 9606.ENSP00000384651

UniGene: Hs.474536

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Autophagy Phosphatidylinositol signaling system

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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