MVD Antibody

Datasheet

Code	CSB-PA015246LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue All lanes: MVD antibody at 7.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa IHC image of CSB-PA015246LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA015246LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MVD Polyclonal antibody

Uniprot No.

P53602

Target Names

MVD

Alternative Names

Diphosphomevalonate decarboxylase antibody; FP17780 antibody; MDDase antibody; Mevalonate (diphospho) decarboxylase antibody; Mevalonate (diphospho)decarboxylase antibody; Mevalonate pyrophosphate decarboxylase antibody; MPD antibody; Mvd antibody; MVD1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Diphosphomevalonate decarboxylase protein (69-221AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MVD Antibody (Product code: CSB-PA015246LA01HU) is Non-conjugated. For MVD Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015246LB01HU	MVD Antibody, HRP conjugated	ELISA
FITC	CSB-PA015246LC01HU	MVD Antibody, FITC conjugated
Biotin	CSB-PA015246LD01HU	MVD Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the ATP dependent decarboxylation of (R)-5-diphosphomevalonate to form isopentenyl diphosphate (IPP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoids and sterol synthesis.

Gene References into Functions

functional importance of R161 and N17 in the binding and orientation of mevalonate diphosphate PMID: 18823933

Involvement in disease

Porokeratosis 7, multiple types (POROK7)

Subcellular Location

Cytoplasm.

Protein Families

Diphosphomevalonate decarboxylase family

Tissue Specificity

Expressed in heart, skeletal muscle, lung, liver, brain, pancreas, kidney and placenta.

Database Links

HGNC: 7529

OMIM: 603236

KEGG: hsa:4597

STRING: 9606.ENSP00000301012

UniGene: Hs.252457

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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