MYH4 Antibody

Datasheet

Code	CSB-PA22719A0Rb
Size	US$166
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Image	IHC image of CSB-PA22719A0Rb diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA22719A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MYH4 Polyclonal antibody

Uniprot No.

Q9Y623

Target Names

MYH4

Alternative Names

fetal antibody; MYH2B antibody; Myh4 antibody; MYH4_HUMAN antibody; MyHC-2b antibody; MyHC-IIb antibody; Myosin heavy chain 2b antibody; Myosin heavy chain 4 antibody; Myosin heavy chain 4 skeletal muscle antibody; Myosin heavy chain antibody; Myosin heavy chain IIb antibody; Myosin heavy chain skeletal muscle fetal antibody; Myosin-4 antibody; skeletal muscle antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Myosin-4 protein (896-1045AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MYH4 Antibody (Product code: CSB-PA22719A0Rb) is Non-conjugated. For MYH4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA22719B0Rb	MYH4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA22719C0Rb	MYH4 Antibody, FITC conjugated
Biotin	CSB-PA22719D0Rb	MYH4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Muscle contraction.

Gene References into Functions

MyHC-2b was always co-expressed with the major MyHC isoforms, not only with the fast ones (MyHC-2a and -2x) but with the slow isoform (MyHC-1) as well. PMID: 23796659

Subcellular Location

Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family

Database Links

HGNC: 7574

OMIM: 160742

KEGG: hsa:4622

STRING: 9606.ENSP00000255381

UniGene: Hs.711141

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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