NAA20 Antibody

Datasheet

Code	CSB-PA015472ESR2HU
Size	US$166
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Image	Western blot All lanes: NAA20 antibody at 10μg/ml Lane 1: Hela whole cell lysate Lane 2: Rat brain tissue Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21, 13 kDa Observed band size: 21 kDa Immunoprecipitating NAA20 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA015472ESR2HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA015472ESR2HU (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NAA20 Polyclonal antibody

Uniprot No.

P61599

Target Names

NAA20

Alternative Names

NAA20 antibody; NAT5 antibody; N-alpha-acetyltransferase 20 antibody; EC 2.3.1.254 antibody; Methionine N-acetyltransferase antibody; N-acetyltransferase 5 antibody; N-terminal acetyltransferase B complex catalytic subunit NAA20 antibody; N-terminal acetyltransferase B complex catalytic subunit NAT5 antibody; NatB complex subunit NAT5 antibody; NatB catalytic subunit antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human N-alpha-acetyltransferase 20 protein (49-178AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration.

Gene References into Functions

The human NatB complex composed of Naa20 (NAT3) and Naa25 (MDM20) N-terminally acetylates Met-Glu-, Met-Asp-, Met-Asn- and Met-Gln- N-termini, and is important for the structure and function of actomyosin fibers and for proper cellular migration. PMID: 22814378
Human N-acetyltransferase complexe NatB consists of human N-acetyltransferase 3 and human MDM20. hNAT3 knockdown results in an increase in G(0)/G(1)-phase cells. PMID: 18570629
hNat3 (NAT5) is the catalytic subunit and hMdm20 the auxiliary subunit of the human NatB N-terminal acetyltransferase complex. This ribosome associated complex acetylates nascent Met-Asp/Glu- polypeptides. PMID: 18570629

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Acetyltransferase family, ARD1 subfamily

Database Links

HGNC: 15908

OMIM: 610833

KEGG: hsa:51126

STRING: 9606.ENSP00000335636

UniGene: Hs.368783

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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