NAA50 Antibody

Datasheet

Code	CSB-PA880928ESR2HU
Size	US$166
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Image	Western blot All lanes: NAA50 antibody at 5ug/ml Lane 1: K562 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20, 10 kDa Observed band size: 20 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NAA50 Polyclonal antibody

Uniprot No.

Q9GZZ1

Target Names

NAA50

Alternative Names

ody; FLJ13194 antibody; hNAT5 antibody; hSAN antibody; Mak3 homolog (S. cerevisiae) antibody; Mak3 homolog antibody; N-acetyltransferase 13 (GCN5-related) antibody; N-acetyltransferase 13 antibody; N-acetyltransferase 5 antibody; N-acetyltransferase san homolog antibody; N-alpha-acetyltransferase 50 antibody; N-alpha-acetyltransferase 50; NatE catalytic subunit antibody; Naa50 antibody; NAA50_HUMAN antibody; NAT 5 antibody; NAT13 antibody; NAT5 antibody; NatE catalytic subunit antibody; San antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human N-alpha-acetyltransferase 50 protein (1-169AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine. Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position. Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins. Autoacetylates in vivo. The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo. Component of N-alpha-acetyltransferase complexes containing NAA10 and NAA15, which has N-alpha-acetyltransferase activity. Does not influence the acetyltransferase activity of NAA10. However, it negatively regulates the N-alpha-acetyltransferase activity of the N-terminal acetyltransferase A complex (also called the NatA complex). The multiprotein complexes probably constitute the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides. Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10.

Gene References into Functions

Because Naa10 is reported to acetylate all amino termini that are devoid of methionine and Naa50 acetylates all other peptides that are followed by methionine PMID: 27484799
co-depletion of NatA, a heterodimeric NAT complex that physically interacts with Naa50, rescues the sister-chromatid cohesion defects and the resulting mitotic arrest caused by Naa50 depletion, indicating that NatA and Naa50 play antagonistic roles in cohesion. PMID: 27422821
The study quantitatively compared the Nt-acetylomes of wild-type yeast S. cerevisiae expressing the endogenous yeast Naa50 (yNaa50), the congenic strain lacking yNaa50, and an otherwise identical strain expressing human Naa50 (hNaa50). PMID: 25886145
Development of the first N-terminal acetyltransferase (NAT) inhibitors, including a specific Naa50 inhibitor. PMID: 23557624
The first description of the human homologue of Nat5p/San, hNAT5, the third component of the human NatA N-alpha-acetyltransferase complex PMID: 16507339
Nat5 displays both protein N alpha- and N epsilon-acetyltransferase activity. PMID: 19744929
Human protein N-terminal acetyltransferase hNaa50p (hNAT5/hSAN) follows ordered sequential catalytic mechanism: combined kinetic and NMR study. PMID: 22311970
Naa50p can accommodate only an alpha-amino substrate and not a side chain lysine substrate that is acetylated by lysine acetyltransferase enzymes such as Gcn5. PMID: 21900231
May be specifically required for the maintenance of centromeric cohesion in mitosis. PMID: 17502424

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Acetyltransferase family, GNAT subfamily

Database Links

HGNC: 29533

OMIM: 610834

KEGG: hsa:80218

STRING: 9606.ENSP00000240922

UniGene: Hs.372378

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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