NDUFAF3 Antibody

Datasheet

Code	CSB-PA880103LA01HU
Size	US$166
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Image	IHC image of CSB-PA880103LA01HU diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA880103LA01HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NDUFAF3 Polyclonal antibody

Uniprot No.

Q9BU61

Target Names

NDUFAF3

Alternative Names

tibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 antibody; NDUF3_HUMAN antibody; ndufaf3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 protein (91-184AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NDUFAF3 Antibody (Product code: CSB-PA880103LA01HU) is Non-conjugated. For NDUFAF3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA880103LB01HU	NDUFAF3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA880103LC01HU	NDUFAF3 Antibody, FITC conjugated
Biotin	CSB-PA880103LD01HU	NDUFAF3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).

Gene References into Functions

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease. PMID: 19463981
Describes a similar transcript in rat, and compares it to human and mouse transcripts. PMID: 9349717
Describes a similar transcript in mouse. PMID: 12653254

Involvement in disease

Mitochondrial complex I deficiency (MT-C1D)

Subcellular Location

Nucleus. Mitochondrion inner membrane.

Database Links

HGNC: 29918

OMIM: 252010

KEGG: hsa:25915

STRING: 9606.ENSP00000323076

UniGene: Hs.31387

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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