NDUFB8 Antibody

Datasheet

Code	CSB-PA015655LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, Rat brain tissue, Mouse heart tissue All lanes: NDUFB8 antibody at 2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22, 21, 19 kDa Observed band size: 22 kDa IHC image of CSB-PA015655LA01HU diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA015655LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NDUFB8 Polyclonal antibody

Uniprot No.

O95169

Target Names

NDUFB8

Alternative Names

NDUFB8; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; Complex I-ASHI; CI-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial protein (29-132AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NDUFB8 Antibody (Product code: CSB-PA015655LA01HU) is Non-conjugated. For NDUFB8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015655LB01HU	NDUFB8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015655LC01HU	NDUFB8 Antibody, FITC conjugated
Biotin	CSB-PA015655LD01HU	NDUFB8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Gene References into Functions

NDUFB8 is a relevant gene in childhood-onset mitochondrial disease. PMID: 29429571
Results describe PTEN and NDUFB8 aberrations in cervical cancer tissue. PMID: 17727244

Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.

Protein Families

Complex I NDUFB8 subunit family

Database Links

HGNC: 7703

OMIM: 602140

KEGG: hsa:4714

STRING: 9606.ENSP00000299166

UniGene: Hs.523215

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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