NDUFB8 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA985048A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), LO2 whole cell lysate(30µg), A431 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), Hela whole cell lysate(30µg), JURKAT whole cell lysate(30µg), A549 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Mouse heart tissue lysate(30µg) All lanes: NDUFB8 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 19 kDa Exposure time：20s IHC image of CSB-RA985048A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA985048A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

O95169

Target Names

NDUFB8

Alternative Names

ASHI antibody; CI-ASHI antibody; Complex I ASHI subunit antibody; Complex I-ASHI antibody; mitochondrial antibody; NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody; NADH-ubiquinone oxidoreductase ASHI subunit antibody; NDUB8_HUMAN antibody; NDUFB8 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human NDUFB8

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Gene References into Functions

NDUFB8 is a relevant gene in childhood-onset mitochondrial disease. PMID: 29429571
Results describe PTEN and NDUFB8 aberrations in cervical cancer tissue. PMID: 17727244

Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.

Protein Families

Complex I NDUFB8 subunit family

Database Links

HGNC: 7703

OMIM: 602140

KEGG: hsa:4714

STRING: 9606.ENSP00000299166

UniGene: Hs.523215

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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