NDUFB8 Recombinant Monoclonal Antibody

NDUFB8 is an accessory subunit of mitochondrial Complex I, the largest enzyme of the oxidative phosphorylation system. As a component of NADH-ubiquinone oxidoreductase, NDUFB8 contributes to the electron transport chain that drives cellular ATP production. Mutations and expression changes in NDUFB8 have been linked to mitochondrial disorders and altered metabolic states, making it a valuable marker for researchers investigating mitochondrial function, bioenergetics, and metabolic disease.

This recombinant monoclonal antibody, generated from clone 8B11 in rabbit host, offers the reproducibility that mitochondrial research demands. Because the antibody sequence is defined and production is cell-line based, you can expect consistent performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation data demonstrates robust performance across multiple experimental platforms. In Western blot applications, the antibody detects NDUFB8 at approximately 19 kDa across a diverse panel of human cell lines including HEK293, A431, PC-3, HeLa, Jurkat, A549, and LO2 cells. The observed molecular weight runs slightly lower than the 22 kDa predicted size, which is consistent with the protein's mitochondrial processing and mature form. Cross-species reactivity extends to mouse samples, with clear detection confirmed in both brain and heart tissue lysates, providing flexibility for researchers working with murine models. Immunohistochemistry validation in human liver cancer and kidney tissue sections demonstrates the antibody's utility for spatial analysis of NDUFB8 expression in tissue contexts.

Whether you're characterizing mitochondrial dysfunction in disease models, profiling Complex I assembly, or examining metabolic reprogramming in cancer, this antibody provides a reliable tool for detecting NDUFB8 across biochemical and histological workflows.