NEDD1 Antibody

Datasheet

Code	CSB-PA822820DSR1HU
Size	US$166
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Image	Western blot All lanes: NEDD1 antibody at 3.25μg/ml Lane 1: Mouse small intestine tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72, 63, 73 kDa Observed band size: 72 kDa Immunohistochemistry of paraffin-embedded human glioma using CSB-PA822820DSR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA822820DSR1HU at dilution of 1:100 Immunoprecipitating NEDD1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA822820DSR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: CSB-PA822820DSR1HU (4μg) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NEDD1 Polyclonal antibody

Uniprot No.

Q8NHV4

Target Names

NEDD1

Alternative Names

FLJ35902 antibody; GCP WD antibody; NEDD-1 antibody; NEDD1 antibody; NEDD1_HUMAN antibody; Neural precursor cell expressed developmentally down-regulated protein 1 antibody; Neural precursor cell expressed, developmentally down regulated gene 1 antibody; Protein NEDD1 antibody; TUBGCP7 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Protein NEDD1 protein (411-660AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Gene References into Functions

One important function of Aurora A in mitotic cells is to promote microtubule nucleation around the chromatin by phosphorylating NEDD1, and thereby to promote functional spindle assembly. PMID: 23273898
The mice treated with small interfering (si) RNA targeting NEDD1 by the atelocollagen-mediated delivery system showed a significantly prolonged survival in a xenograft model of human gastric cancer. PMID: 23106787
Data suggest that the NEDD1/gamma-tubulin interaction is finely tuned by multiple phosphorylation events in the S557-S574 region and is critical for spindle assembly. PMID: 22595525
a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
GCP-WD (NEDD1) broadly mediates targeting of the gamma-tubulin ring complex to sites of microtubule nucleation and to the mitotic spindle, which is essential for spindle formation. PMID: 16378099
The NEED1 protein was characterized and its function in centriole duplication and spindle assembly determined. PMID: 16461362
Data propose NEDD1 may be a promising target for controlling cell proliferation, in particular if targeted in combination with Plk1 inhibitors. PMID: 19243593
Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Database Links

HGNC: 7723

OMIM: 600372

KEGG: hsa:121441

STRING: 9606.ENSP00000451211

UniGene: Hs.270084

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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