NETO1 Antibody

Datasheet

Code	CSB-PA840571LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, U87 whole cell lysate All lanes: NETO1 antibody at 3.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 18 kDa Observed band size: 61 kDa Immunofluorescence staining of HepG2 cells with CSB-PA840571LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NETO1 Polyclonal antibody

Uniprot No.

Q8TDF5

Target Names

NETO1

Alternative Names

AI851453 antibody; BCTL1 antibody; Brain specific transmembrane protein containing 2 CUB and 1 LDL receptor class A domains protein 1 antibody; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 antibody; BTCL1 antibody; C130005O10Rik antibody; FLJ41325 antibody; Neto1 antibody; NETO1_HUMAN antibody; Neuropilin and tolloid like 1 antibody; Neuropilin and tolloid-like protein 1 antibody; RGD1566269 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Neuropilin and tolloid-like protein 1 protein (367-507AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NETO1 Antibody (Product code: CSB-PA840571LA01HU) is Non-conjugated. For NETO1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA840571LB01HU	NETO1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA840571LC01HU	NETO1 Antibody, FITC conjugated
Biotin	CSB-PA840571LD01HU	NETO1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Enhanced NRP1 Expression in Dendritic Cells of Systemic Lupus Erythematosus and Its Impact on T Cell Proliferation Y Choi,researchsquare,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Western blot

Sample type: cell

Review: NRP1 expression of PBMCs compared between healthy controls (n=60) and patients with SLE (n=57).

By Anonymous

Target Background

Function

Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.

Gene References into Functions

Neto1 is concluded to be a component of amyloid precursor protein /NMDA receptor trafficking complexes PMID: 23621516
no evidence for the association of NETO1 with schizophrenia in the Japanese population PMID: 22205981
Mouse neto1 plays a critical role in spatial learning and memory through the regulation of NMDAR subunit abundance at synapses. PMID: 19243221

Subcellular Location

[Isoform 2]: Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic density membrane.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein.; [Isoform 1]: Secreted.

Tissue Specificity

Isoform 1 and isoform 2 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain.

Database Links

HGNC: 13823

OMIM: 607973

KEGG: hsa:81832

STRING: 9606.ENSP00000313088

UniGene: Hs.465407

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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