NEU4 Antibody

Code CSB-PA848831LA01HU
Size US$166
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Image
  • IHC image of CSB-PA848831LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA848831LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA848831LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NEU4 Polyclonal antibody
Uniprot No.
Target Names
NEU4
Alternative Names
NEU4 antibody; LP5125Sialidase-4 antibody; EC 3.2.1.18 antibody; N-acetyl-alpha-neuraminidase 4 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Sialidase-4 protein (225-377AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The NEU4 Antibody (Product code: CSB-PA848831LA01HU) is Non-conjugated. For NEU4 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA848831LB01HU NEU4 Antibody, HRP conjugated ELISA
FITC CSB-PA848831LC01HU NEU4 Antibody, FITC conjugated
Biotin CSB-PA848831LD01HU NEU4 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Hydrolyzes poly-alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons. May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation. Has sialidase activity toward mucin, fetuin and sialyllactose.
Gene References into Functions
  1. The most potent compound tested targeted the human Neu4 isoenzyme, and was able to substantially reduce the rate of cell migration. We found that the lateral mobility of integrins was reduced by treatment of cells with Neu3, suggesting that Neu3 enzyme activity resulted in changes to integrin-co-receptor or integrin-cytoskeleton interactions PMID: 27344026
  2. The silencing or chemical inhibition of NEU4 changes the entire glycosylation pattern of proteins and lipids, making it more similar to that of differentiated GBM cells and drastically reduces GSCs survival. PMID: 25144716
  3. The results demonstrate that the proline-rich region can also enhance cell proliferation and retinoic acid (RA)-induced neuronal differentiation and it is also involved in NEU4 interaction with Akt. PMID: 24030392
  4. Overexpression of NEU4 is associated with neuroblastoma. PMID: 22287118
  5. NEU4 is involved in regulation of neuronal function by polySia degradation in mammals. PMID: 22393058
  6. NEU4 plays an important role in control of sialyl Lewis antigen expression and its impairment in colon cancer PMID: 21521691
  7. The NEU4 long form localizes in mitochondria, while the short form is also associated with the endoplasmic reticulum. PMID: 19797320
  8. The NEU4 gene, identified by searching sequence databases for entries showing homologies to the human cytosolic sialidase NEU2, maps in 2q37 and encodes a 484-residue protein. PMID: 14962670
  9. Neu4 is a novel human lysosomal lumen sialidase PMID: 15213228
  10. Data show that the differentiation of monocytes into macrophages is associated with regulation of the expression of at least three distinct cellular sialidases, Neu1, 3, and 4, with specific up-regulation of the enzyme activity of only Neu1. PMID: 15885103
  11. related to malignancy and may be potential targets for cancer diagnosis and therapy PMID: 18651674

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Subcellular Location
[Isoform 1]: Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Cell projection, neuron projection.; [Isoform 2]: Mitochondrion inner membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Lysosome lumen. Note=According to PubMed:15213228, isoform 2 is soluble, N-glycosylated and found in the lumen of lysosomes. However, no signal sequence nor N-glycosylation site is predicted from the sequence.
Protein Families
Glycosyl hydrolase 33 family
Tissue Specificity
[Isoform 1]: Predominant form in liver. Also expressed in brain, kidney and colon.; [Isoform 2]: Highly expressed in brain and at lower levels in kidney and liver.
Database Links

HGNC: 21328

OMIM: 608527

KEGG: hsa:129807

STRING: 9606.ENSP00000320318

UniGene: Hs.551747

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