NFE2L3 Antibody

Code CSB-PA896721LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate
    All lanes: NFE2L3 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 77 kDa
    Observed band size: 77 kDa

  • IHC image of CSB-PA896721LA01HU diluted at 1:500 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NFE2L3 Polyclonal antibody
Uniprot No.
Target Names
NFE2L3
Alternative Names
NFE2L3 antibody; NRF3 antibody; Nuclear factor erythroid 2-related factor 3 antibody; NF-E2-related factor 3 antibody; NFE2-related factor 3 antibody; Nuclear factor antibody; erythroid derived 2 antibody; like 3 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Nuclear factor erythroid 2-related factor 3 protein (132-260AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The NFE2L3 Antibody (Product code: CSB-PA896721LA01HU) is Non-conjugated. For NFE2L3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA896721LB01HU NFE2L3 Antibody, HRP conjugated ELISA
FITC CSB-PA896721LC01HU NFE2L3 Antibody, FITC conjugated
Biotin CSB-PA896721LD01HU NFE2L3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Activates erythroid-specific, globin gene expression.
Gene References into Functions
  1. SNPs associated with type 2 diabetes and obesity may also increase the risk of developing gestational diabetes mellitus (GDM) in the Chinese population. Among these SNPs, we report for the first time that rs945508 in ARHGEF11, rs10804591 in PLXND1 and rs10245353 in NFE2L3 were associated with GDM. PMID: 28554271
  2. findings reveal FBW7 and GSK3 as novel regulators of the NFE2L3 transcription factor and a potential mechanism by which FBW7 might regulate detoxification and the cellular response to stress PMID: 26306035
  3. 17beta-Estradiol treatment significantly increased but VC or Res decreased Nrf3 mRNA and protein expression levels. PMID: 25130429
  4. Nrf3 is a negative regulator of ARE-mediated gene expression of NQO1 PMID: 15385560
  5. NRF3 is highly expressed in primary placental cytotrophoblasts PMID: 15388789
  6. Nrf3 is an N-glycosylated protein associated with the endoplasmic reticulum PMID: 17976382

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Subcellular Location
Nucleus.
Protein Families
BZIP family, CNC subfamily
Tissue Specificity
Highly expressed in human placenta and also in B-cell and monocyte cell lines. Low expression in heart, brain, lung, skeletal muscle, kidney and pancreas.
Database Links

HGNC: 7783

OMIM: 604135

KEGG: hsa:9603

STRING: 9606.ENSP00000056233

UniGene: Hs.404741

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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