Product Details

Uniprot No.

Target Names

NOLC1

Alternative Names

140 kDa nucleolar phosphoprotein antibody; HCV NS5A trans regulated protein 13 antibody; HCV NS5A transactivated protein 13 antibody; HCV NS5A-transactivated protein 13 antibody; Hepatitis C virus NS5A transactivated protein 13 antibody; Hepatitis C virus NS5A-transactivated protein 13 antibody; KIAA0035 antibody; NOLC 1 antibody; NOLC1 antibody; NOLC1_HUMAN antibody; NOPP 130 antibody; NOPP 140 antibody; NOPP130 antibody; Nopp140 antibody; NS5ATP13 antibody; Nucleolar 130 kDa protein antibody; Nucleolar and coiled body phosphoprotein 1 antibody; Nucleolar and coiled body phosphprotein 1 antibody; Nucleolar and coiled-body phosphoprotein 1 antibody; Nucleolar phosphoprotein p130 antibody; Nucleolar protein p130 antibody; P130 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from the C-terminal region of Human Nopp140.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:40000

Protocols

Western Blotting(WB) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities.

Gene References into Functions

In this study, the disordered feature of Nopp140 and the effect of CK2alpha on the structure of Nopp140 were examined using single-molecule fluorescence resonance energy transfer (smFRET) and electron paramagnetic resonance (EPR). PMID: 27297113
D120 and R195 of H5N1 influenza virus NS1 were crucial for the binding to host NOLC1. PMID: 25645906
pRb2/p130 cytoplasmic delocalization can lead to cell cycle deregulation PMID: 25205458
our study demonstrated that DNA methylation is a key mechanism of silenced NOLC1 expression in human hepatocellular carcinoma cells, and NOLC1 gene hypermethylation of the four CpG dinucleotides is a potential biomarker for hepatocellular carcinoma. PMID: 23970161
A Nopp140 fragment (residues 568-596) and inositol hexakisphosphate competitively bind to the catalytic subunit of CK2 (CK2alpha), and phospho-Ser574 of Nopp140 significantly enhances its interaction with CK2alpha. PMID: 24218616
H5N1 viral NS1 interacts with host NOLC1 protein. PMID: 23188192
Structural disorder and local order of hNopp140 PMID: 22906532
The regulation of NOCL1 protein is a critical role for the transcription NF-kB and cAMP response element-bing protein PMID: 21266110
NOPP140 is involved in a PKA signaling pathway that leads to agp activation PMID: 12167624
These data show that Nopp140 reversibly associates with snoRNPs in a phosphorylation-dependent manner, but this association has no effect on pseudouridylation of rRNA. PMID: 12446766
Hypoxia induces substantial p130 dephosphorylation and nuclear accumulation, leading to the formation of E2F4/p130 complexes and increased occupancy of E2F4 and p130 at the RAD51 and BRCA1 promoters. PMID: 17001309
hNopp140 serves as a negative regulator of CK2 and InsP(6) stimulates the activity of CK2 by blocking the interaction between hNopp140 and CK2 PMID: 17038328
Evolutionarily conserved multisubunit protein complex that contains p130 and E2F4 mediates the repression of cell cycle-dependent genes in quiescence. PMID: 17531812
hNopp140, a scaffold protein, is involved in the nucleolar assembly, fusion, and maintenance PMID: 18253863
InsP(6) specifically binds to CK2alpha and disrupts the interaction between CK2alpha and Nopp140 with an IC(50) value of 25 microM, thereby attenuating the Nopp140-mediated repression of CK2 activity. PMID: 18790693
The functional deficiency in spinal muscular atrophy cells is associated with decreased localization of the snoRNP chaperone Nopp140 in Cajal bodies that correlates with disease severity. PMID: 19129172
Nopp140 could be a component implicated in the early steps of pre-rRNA processing. PMID: 19381672
both NOLC1 and tumor protein 53 work together synergistically to activate the MDM2 promoter in NPC cells. PMID: 19541936