NOLC1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA027798A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), A549 whole cell lysate(30µg) All lanes: NOLC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 74 kDa Observed band size: 110 kDa Exposure time：1min IHC image of CSB-RA027798A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA027798A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA027798A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q14978

Target Names

NOLC1

Alternative Names

140 kDa nucleolar phosphoprotein antibody; HCV NS5A trans regulated protein 13 antibody; HCV NS5A transactivated protein 13 antibody; HCV NS5A-transactivated protein 13 antibody; Hepatitis C virus NS5A transactivated protein 13 antibody; Hepatitis C virus NS5A-transactivated protein 13 antibody; KIAA0035 antibody; NOLC 1 antibody; NOLC1 antibody; NOLC1_HUMAN antibody; NOPP 130 antibody; NOPP 140 antibody; NOPP130 antibody; Nopp140 antibody; NS5ATP13 antibody; Nucleolar 130 kDa protein antibody; Nucleolar and coiled body phosphoprotein 1 antibody; Nucleolar and coiled body phosphprotein 1 antibody; Nucleolar and coiled-body phosphoprotein 1 antibody; Nucleolar phosphoprotein p130 antibody; Nucleolar protein p130 antibody; P130 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human NOLC1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1D9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NOLC1, also known as Nopp140, serves as a critical nucleolar phosphoprotein that shuttles between the nucleolus and cytoplasm, playing essential roles in ribosome biogenesis and the structural organization of Cajal bodies. This protein functions as a chaperone for small nucleolar ribonucleoproteins and has been implicated in various cellular processes including cell cycle regulation and responses to cellular stress. Its involvement in nucleolar dynamics makes NOLC1 a valuable target for researchers investigating ribosome assembly, nuclear architecture, and the emerging connections between nucleolar function and disease states.

This recombinant monoclonal antibody, generated in rabbit using clone 1D9, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant technology, researchers can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis in HEK293, MCF-7, and A549 cell lysates reveals detection at approximately 110 kDa, notably higher than the predicted 74 kDa molecular weight. This size difference likely reflects the extensive post-translational modifications characteristic of NOLC1, particularly its heavy phosphorylation and potential glycosylation. Immunohistochemistry validation in human colorectal cancer tissue sections confirms utility for tissue-based studies, while immunofluorescence staining in HeLa cells and flow cytometry analysis in HepG2 cells demonstrate robust performance for cellular localization and quantitative single-cell applications.

This antibody supports investigations in epigenetics and nuclear signaling, providing researchers studying nucleolar biology and related pathways with a dependable detection tool.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities.

Gene References into Functions

In this study, the disordered feature of Nopp140 and the effect of CK2alpha on the structure of Nopp140 were examined using single-molecule fluorescence resonance energy transfer (smFRET) and electron paramagnetic resonance (EPR). PMID: 27297113
D120 and R195 of H5N1 influenza virus NS1 were crucial for the binding to host NOLC1. PMID: 25645906
pRb2/p130 cytoplasmic delocalization can lead to cell cycle deregulation PMID: 25205458
our study demonstrated that DNA methylation is a key mechanism of silenced NOLC1 expression in human hepatocellular carcinoma cells, and NOLC1 gene hypermethylation of the four CpG dinucleotides is a potential biomarker for hepatocellular carcinoma. PMID: 23970161
A Nopp140 fragment (residues 568-596) and inositol hexakisphosphate competitively bind to the catalytic subunit of CK2 (CK2alpha), and phospho-Ser574 of Nopp140 significantly enhances its interaction with CK2alpha. PMID: 24218616
H5N1 viral NS1 interacts with host NOLC1 protein. PMID: 23188192
Structural disorder and local order of hNopp140 PMID: 22906532
The regulation of NOCL1 protein is a critical role for the transcription NF-kB and cAMP response element-bing protein PMID: 21266110
NOPP140 is involved in a PKA signaling pathway that leads to agp activation PMID: 12167624
These data show that Nopp140 reversibly associates with snoRNPs in a phosphorylation-dependent manner, but this association has no effect on pseudouridylation of rRNA. PMID: 12446766
Hypoxia induces substantial p130 dephosphorylation and nuclear accumulation, leading to the formation of E2F4/p130 complexes and increased occupancy of E2F4 and p130 at the RAD51 and BRCA1 promoters. PMID: 17001309
hNopp140 serves as a negative regulator of CK2 and InsP(6) stimulates the activity of CK2 by blocking the interaction between hNopp140 and CK2 PMID: 17038328
Evolutionarily conserved multisubunit protein complex that contains p130 and E2F4 mediates the repression of cell cycle-dependent genes in quiescence. PMID: 17531812
hNopp140, a scaffold protein, is involved in the nucleolar assembly, fusion, and maintenance PMID: 18253863
InsP(6) specifically binds to CK2alpha and disrupts the interaction between CK2alpha and Nopp140 with an IC(50) value of 25 microM, thereby attenuating the Nopp140-mediated repression of CK2 activity. PMID: 18790693
The functional deficiency in spinal muscular atrophy cells is associated with decreased localization of the snoRNP chaperone Nopp140 in Cajal bodies that correlates with disease severity. PMID: 19129172
Nopp140 could be a component implicated in the early steps of pre-rRNA processing. PMID: 19381672
both NOLC1 and tumor protein 53 work together synergistically to activate the MDM2 promoter in NPC cells. PMID: 19541936

Hide All

Subcellular Location

Nucleus, nucleolus. Cytoplasm.

Database Links

HGNC: 15608

OMIM: 602394

KEGG: hsa:9221

STRING: 9606.ENSP00000359024

UniGene: Hs.523238

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address