NOXO1 Antibody

Datasheet

Code	CSB-PA015964LA01HU
Size	US$166
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Image	IHC image of CSB-PA015964LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA015964LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA015964LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NOXO1 Polyclonal antibody

Uniprot No.

Q8NFA2

Target Names

NOXO1

Alternative Names

NADPH oxidase organizer 1 antibody; NADPH oxidase regulatory protein antibody; Nox organizer 1 antibody; Nox organizing protein 1 antibody; Nox-organizing protein 1 antibody; NOXO1 antibody; NOXO1_HUMAN antibody; P41NOX antibody; Regulatory protein P41NOX antibody; SH3 and PX domain-containing protein 5 antibody; SH3PXD5 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human NADPH oxidase organizer 1 protein (304-376AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NOXO1 Antibody (Product code: CSB-PA015964LA01HU) is Non-conjugated. For NOXO1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015964LB01HU	NOXO1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015964LC01HU	NOXO1 Antibody, FITC conjugated
Biotin	CSB-PA015964LD01HU	NOXO1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.

Gene References into Functions

PEDF protects human glomerular mesangial cells from diabetes-derived oxidative stress via NOXO1- iNOS suppression. PMID: 28944893
Data from pull-down assay between the Noxo1 and Noxa1 showed that the SH3 domains (Noxa1) is responsible for interaction with Noxo1 C-terminal tail harboring proline rich region. PMID: 28625920
TNF-alpha/TNFR1 signaling promotes gastric tumorigenesis through induction of Noxo1 and Gna14 in tumor cells. PMID: 23975421
Phosphorylation of Thr341 allows Noxo1 to sufficiently interact with Noxa1, an interaction that participates in Nox1 activation PMID: 23957209
Phorbol myristate acetate stimulates NOXO1 phosphorylation in a transfected human embryonic kidney (HEK) 293 epithelial cell model via protein kinase C and identify Ser-154 as the major phosphorylated site. PMID: 23322165
Surface plasmon resonance experiments identify PtdIns(4,5)P(2) and PtdIns(3,4,5)P(3) as preferred targets of NOXO1beta PX. PMID: 22342885
The backbone assignments of NOXO1beta PX are studied using NMR. PMID: 21188560
The first quantitative characterization of the interactions made between the cytosolic regulators NOXO1 and NOXA1 and membrane-bound p22(phox), is presented. PMID: 20454568
NOXO1 and NOXA1 activate Nox1 without the need for agonist activation, and this is mediated in part by binding of the NOXO1 PX domain to membrane lipids. PMID: 14617635
NOXO1, p47phox, and p67phox regulate Nox3 PMID: 15181005
Data suggest different tissue localizations and functions for NOXO1beta and NOXO1gamma in regulating Nox family members. PMID: 15949904
These results suggest that the formation of the complex consisting of Nox1, betaPix, and NoxO1 is likely to be a critical step in EGF-induced ROS generation. PMID: 16329988
This study shows the expression of alternatively spliced transcripts of the NOXO1 gene and the roles of the protein products in the activation of Nox oxidases. PMID: 16911517
These findings suggest that Nox activation involves a conformational change leading to disruption of the bis-SH3-PRR interaction in Noxo1. PMID: 17126813

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Subcellular Location

[Isoform 3]: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Isoform 3 associates with the plasma membrane in a lipid-dependent manner (PubMed:12716910).

Tissue Specificity

Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.

Database Links

HGNC: 19404

OMIM: 611256

KEGG: hsa:124056

STRING: 9606.ENSP00000380450

UniGene: Hs.191762

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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