OLA1 Antibody

Datasheet

Code	CSB-PA016318HA01HU
Size	US$166
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Image	Western blot All lanes: OLA1 antibody at 2µg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 28, 32 kDa Observed band size: 45 kDa Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA016318HA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA016318HA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA016318HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) OLA1 Polyclonal antibody

Uniprot No.

Q9NTK5

Target Names

OLA1

Alternative Names

DKFZp313H1942 antibody; DNA damage-regulated overexpressed in cancer 45 protein antibody; DOC45 antibody; EC 3.6.3 antibody; GBP45 antibody; GTP-binding protein 9 (putative) antibody; GTP-binding protein 9 antibody; GTP-binding protein PTD004 antibody; homologous yeast-44.2 protein antibody; Obg like ATPase 1 antibody; Obg-like ATPase 1 antibody; OLA1 antibody; OLA1_HUMAN antibody; PTD004 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Obg-like ATPase 1 protein (1-396AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The OLA1 Antibody (Product code: CSB-PA016318HA01HU) is Non-conjugated. For OLA1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA016318HB01HU	OLA1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA016318HC01HU	OLA1 Antibody, FITC conjugated
Biotin	CSB-PA016318HD01HU	OLA1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.

Gene References into Functions

No germline mutations were found in the OLA1 gene in in patients with BRCA1/2 mutation-negative suspected hereditary breast and ovarian cancer. PMID: 27271530
OLA1 contributes to epithelial-mesenchymal transition by modulating the GSK3beta/Snail/E-cadherin signaling, and its overexpression is associated with clinical progression and poor survival in lung cancer patients. PMID: 26863455
Our work suggests that OLA1 is a novel translational GTPase and plays a suppressive role in translation and cell survival, as well as cancer growth and progression. PMID: 26283179
OLA1 plays an important negative role in cell adhesion and spreading, in part through the regulation of FAK expression and cofilin phosphorylation, and manipulation of OLA1 may lead to significant changes in cell adhesion and the associated phenotypes. PMID: 24486488
OLA1 plays an important role in centrosome regulation together with BRCA1. PMID: 24289923
Downregulation of OLA1 in mammalian cells by either RNAi or targeted gene disruption results in reduced steady-state levels of HSP70, impaired HSP70 induction by heat, and functionally, increased cellular sensitivity to heat shock. PMID: 23412384
DOC45 is a novel ATPase that is linked to cellular stress response and tumorigenesis, and may also serve as a valuable tumor marker. PMID: 20053727
knockdown of OLA1 inhibits breast cancer cell migration and invasion through a mechanism that involves the modulation of intracellular ROS levels PMID: 19882753
Results indicate that Gbp45/OLA1 plays important roles in cell proliferation and death related to mitochondrial function. PMID: 18536999
OLA1 suppresses the antioxidant response through nontranscriptional mechanisms. PMID: 19706404

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, nucleolus.

Protein Families

TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family, YchF/OLA1 subfamily

Tissue Specificity

Expressed in all tissues tested but its expression is more abundant in testis, liver, lung, and brain. Overexpressed in several malignancies, including cancers of the colon, rectum, ovary, lung, stomach, and uterus.

Database Links

HGNC: 28833

OMIM: 611175

KEGG: hsa:29789

STRING: 9606.ENSP00000284719

UniGene: Hs.157351

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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