PACS2 Antibody

Datasheet

Code	CSB-PA017372LA01HU
Size	US$166
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Image	IHC image of CSB-PA017372LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA017372LA01HU diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PACS2 Polyclonal antibody

Uniprot No.

Q86VP3

Target Names

PACS2

Alternative Names

6720425G15Rik antibody; AW208651 antibody; AW489976 antibody; E230011J22 antibody; FLJ25488 antibody; KIAA0602 antibody; mKIAA0602 antibody; PACS 2 antibody; PACS-2 antibody; PACS1 like protein antibody; PACS1-like protein antibody; PACS1L antibody; PACS2 antibody; PACS2_HUMAN antibody; Phosphofurin acidic cluster sorting protein 1 like antibody; Phosphofurin acidic cluster sorting protein 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Phosphofurin acidic cluster sorting protein 2 protein (268-465AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PACS2 Antibody (Product code: CSB-PA017372LA01HU) is Non-conjugated. For PACS2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017372LB01HU	PACS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA017372LC01HU	PACS2 Antibody, FITC conjugated
Biotin	CSB-PA017372LD01HU	PACS2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : IP

Sample type: Human Human neuroblastoma cell

Review: Knockdown of bcl-2 was done using siRNA transfection for 24 h and then cells were treated with cortisol (1 μM) during 2 h. mfn2 was co-immunoprecipitated with an anti-PACS2 antibody (the left side). Expression of PACS2, mfn2, and β-actin in total cell lysates is shown in the right side.

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Target Background

Function

Multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, including the apposition of mitochondria with the ER and ER homeostasis. In addition, in response to apoptotic inducer, translocates BIB to mitochondria, which initiates a sequence of events including the formation of mitochondrial truncated BID, the release of cytochrome c, the activation of caspase-3 thereby causing cell death. May also be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.

Gene References into Functions

These findings support the causality of this recurrent de novo PACS2 heterozygous missense in Developmental and epileptic encephalopathies with facial dysmorphim and cerebellar dysgenesis. PMID: 29656858
Protein adaptation and the expanding roles of the PACS1 and PACS2 proteins in tissue homeostasis and disease has been summarized. (Review) PMID: 28476937
Missense Mutation in PACS2 gene is associated with Neurodevelopmental Disorders. PMID: 28867141
cIAPs constitutively downregulate PACS-2 by polyubiquitination and proteasomal degradation, thereby restraining TRAIL-induced killing of liver cancer cells PMID: 24633224
The sites on Nef and the PACS proteins required for their interaction, are identified. PMID: 22496420
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein-induced lysosomal translocation of proapoptotic effectors is mediated by phosphofurin acidic cluster sorting protein-2. PMID: 22645134
subcellular localization and function of polycystin-2 are directed by phosphofurin acidic cluster sorting protein (PACS)-1 and PACS-2 PMID: 15692563
PACS-2 as a novel sorting protein that links the endoplasmic reticulum (ER)-mitochondria axis to ER homeostasis PMID: 15692567
PACS-2 is required for Nef action and sorting of itinerant membrane cargo in the TGN/endosomal system PMID: 18296443
the phosphorylation state of the calnexin cytosolic domain and its interaction with PACS-2 sort the chaperone between domains of the ER and the plasma membrane PMID: 18417615
Results identify PACS-2 as an essential TRAIL effector, and show that Akt cooperates with 14-3-3 to regulate the homeostatic and apoptotic properties of PACS-2 that mediate TRAIL action. PMID: 19481529

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Subcellular Location

Endoplasmic reticulum. Mitochondrion.

Protein Families

PACS family

Tissue Specificity

Broadly expressed, with greatest levels in skeletal muscle followed by heart, brain, pancreas and testis.

Database Links

HGNC: 23794

OMIM: 610423

KEGG: hsa:23241

STRING: 9606.ENSP00000393559

UniGene: Hs.525626

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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