PARP8 Antibody

Datasheet

Code	CSB-PA847623LA01HU
Size	US$166
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Image	IHC image of CSB-PA847623LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA847623LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PARP8 Polyclonal antibody

Uniprot No.

Q8N3A8

Target Names

PARP8

Alternative Names

ADP ribosyltransferase diphtheria toxin like 16 antibody; ARTD16 antibody; FLJ21308 antibody; MGC42864 antibody; PARP-8 antibody; Parp8 antibody; PARP8_HUMAN antibody; pART16 antibody; Poly (ADP ribose) polymerase family member 8 antibody; Poly [ADP-ribose] polymerase 8 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Poly [ADP-ribose] polymerase 8 protein (628-854AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PARP8 Antibody (Product code: CSB-PA847623LA01HU) is Non-conjugated. For PARP8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA847623LB01HU	PARP8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA847623LC01HU	PARP8 Antibody, FITC conjugated
Biotin	CSB-PA847623LD01HU	PARP8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.

Database Links

HGNC: 26124

KEGG: hsa:79668

UniGene: Hs.369581

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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