PDE2A Antibody

Datasheet

Code	CSB-PA017687LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate All lanes: PDE2A antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 106, 104, 105, 79, 88 kDa Observed band size: 88 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA017687LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA017687LA01HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA017687LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PDE2A Polyclonal antibody

Uniprot No.

O00408

Target Names

PDE2A

Alternative Names

5''-cyclic phosphodiesterase antibody; cGMP dependent 3'5' cyclic phosphodiesterase antibody; cGMP stimulated phosphodiesterase 1 antibody; cGMP stimulated phosphodiesterase 4 antibody; cGMP-dependent 3'' antibody; cGMP-stimulated phosphodiesterase 2 antibody; CGS PDE antibody; CGS-PDE antibody; cGSPDE antibody; Cyclic GMP stimulated phosphodiesterase antibody; Cyclic GMP-stimulated phosphodiesterase antibody; Human cGMP stimulated 3'5' cyclic nucleotide phosphodiesterase antibody; PDE 2A antibody; PDE 2A1 antibody; PDE 2A4 antibody; PDE2A antibody; PDE2A_HUMAN antibody; PDE2A1 antibody; PDE2A4 antibody; phosphodiesterase 2A antibody; Phosphodiesterase 2A cGMP stimulated antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human cGMP-dependent 3\\\',5\\\'-cyclic phosphodiesterase protein (15-226AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PDE2A Antibody (Product code: CSB-PA017687LA01HU) is Non-conjugated. For PDE2A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017687LB01HU	PDE2A Antibody, HRP conjugated	ELISA
FITC	CSB-PA017687LC01HU	PDE2A Antibody, FITC conjugated
Biotin	CSB-PA017687LD01HU	PDE2A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line).; Regulates mitochondrial cAMP levels and respiration. Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L.

Gene References into Functions

the well-known interaction between AIP and 2 different isoforms of phosphodiesterases (PDEs), PDE2A3 and PDE4A5, is of particular interest. While the interaction with over-expressed AIP does not seem to affect PDE2A3 function, the reported effect on PDE4A5 is, in contrast, reduced enzymatic activity. PMID: 28427099
Lu AF33241 represents a novel PDE2A/PDE10A inhibitor tool which can penetrate the blood brain barrier. PMID: 25941078
Epigenetic regulation of phosphodiesterases 2A and 3A underlies compromised beta-adrenergic Signaling in an induced pluripotent stem cell model of dilated cardiomyopathy. PMID: 26095046
curcumin exerts its in vitro anti-angiogenic and in vivo anti-tumour properties through combined PDE2 and PDE4 inhibition PMID: 25230992
PDE2A is involved in growth and invasion of human malignant melanoma PMP cells. PMID: 24705027
This study reports the X-ray crystal structure of PDE2A in complex with a highly selective, nanomolar inhibitor (BAY60-7550) at 1.9 A resolution, and the structure of apo PDE2 at 2.0 A resolution. PMID: 23899287
PDE2 overexpression in healthy cardiomyocytes reduces the rise in cyclic AMP levels and L-type calcium current amplitude, and abolishes the inotropic effect following acute beta-adrenergic receptor stimulation. PMID: 23810893
Our results demonstrated that the PDE2A2 variant carrying point mutations is expressed in PMP cells and may affect cell cycle progression by modulating cyclin A expression. PMID: 23381931
found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
Active site similarity between human and Plasmodium falciparum phosphodiesterases: considerations for antimalarial drug design PMID: 21766240
the phosphodiesterase 2A isoform localized to mitochondria regulates respiration PMID: 21724846
Inhibition of PDE2 resulted in higher levels of intracellular cAMP than inhibition of PDE3A suggesting this PDE may be the more important regulator of cAMP in human platelets. PMID: 12038792
TNF-alpha-mediated up-regulation of PDE2 may destabilize endothelial barrier function in sepsis PMID: 15650061
Phosphodiesterase 2 mediates redox-sensitive endothelial cell proliferation and angiogenesis by thrombin via Rac1 and NADPH oxidase 2. PMID: 19390057
report the X-ray crystal structures of a PDE protein that includes both catalytic and regulatory domains, namely, PDE2A containing the N-terminal GAF domains and the catalytic domain. PMID: 19828435

Hide All

Subcellular Location

[Isoform PDE2A3]: Cell membrane; Lipid-anchor.; [Isoform PDE2A2]: Mitochondrion matrix. Mitochondrion inner membrane. Mitochondrion outer membrane.; [Isoform PDE2A1]: Cytoplasm.; [Isoform 5]: Mitochondrion.

Protein Families

Cyclic nucleotide phosphodiesterase family, PDE2 subfamily

Tissue Specificity

Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.

Database Links

HGNC: 8777

OMIM: 602658

KEGG: hsa:5138

STRING: 9606.ENSP00000334910

UniGene: Hs.503163

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Nitric oxide signaling cGMP-PKG signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address